LOCUS       sequence-156350-       11720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FUGW lentiviral vector with constitutively expressed eGFP regulated 
            by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor 
            reported in manuscript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Toneff MJ, Sreekumar A, Tinnirello A, Hollander PD, Habib S, Li S, 
            Ellis MJ, Xin L, Mani SA, Rosen JM
  TITLE     The Z-cad dual fluorescent sensor detects dynamic changes between 
            the epithelial and mesenchymal cellular states.
  JOURNAL   BMC Biol. 2016 Jun 17;14(1):47.
  PUBMED    27317311
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-06-17"; volume: "14(1)"; pages: "47"
COMMENT     Sequence Label: FUGW-eGFP-ZEB1 3'UTR
FEATURES             Location/Qualifiers
     source          1..11720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        241..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        622..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     775..795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             838..1018
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1065..1190
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2614..3824
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3661..3680
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3872..3881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3878..3880,3881..3883,3884..4597)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3878..3880,3881..3883,3884..4594)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3923..3944)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4184..4203)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4531..4552
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6412..7000
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6465..6485)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6883..6894)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7003..7019)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7004..7020)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7525..7705
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7731..7748)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7737..7961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8007..8435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8094..8113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8304..8325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8444..8464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8449..8778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8629..8764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8691..8710
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8826..8873
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8892..9266
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9396..9517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9433..9452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9487..9506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9566..9582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9566..9582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9579..9601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9590..9606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9614..9620,9621..9638,9639..9644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9659..9680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9797..9814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9968..10556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10048..10067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10727..11518,11519..11587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11350..11369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11588..11692)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gncgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
       61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      841 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      901 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      961 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1021 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1081 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1141 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1201 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1261 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1321 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1381 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1441 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1501 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttaa ttaagggtgc agcggcctcc gcgccgggtt ttggcgcctc
     2641 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcaggagcg
     2701 ttcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     2761 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     2821 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     2881 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     2941 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     3001 atcgtcactt ggtgagttgc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     3061 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     3121 aaggttgccc tgaactgggg gttgggggga gcgcacaaaa tggcggctgt tcccgagtct
     3181 tgaatggaag acgcttgtaa ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     3241 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
     3301 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
     3361 ctcgggtttg tcgtctggtt gcgggggcgg cagttatgcg gtgccgttgg gcagtgcacc
     3421 cgtacctttg ggagcgcgcg cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
     3481 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
     3541 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
     3601 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
     3661 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
     3721 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
     3781 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct tgggctgcag
     3841 gtcgactcta gaggatcccc gggtaccggt cgccaccatg gtgagcaagg gcgaggagct
     3901 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3961 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4021 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     4081 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4141 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4201 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4261 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4321 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4381 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4441 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     4501 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4561 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagaattcc
     4621 tagtcaaaat aaatccgggt gtgcctgaac ctcagaccta gtaatttttc atgcagtttt
     4681 caaagttagg aacaagtttg tgacatgcag cagattagaa aaccttaatg actcagagag
     4741 caacaataca agaggttaaa ggaagctgat taattagata tgcatctggc attgttttat
     4801 cttatcagta ttatcactct tatgttggtt tattcttaag ctgtacaatt gggagaaatt
     4861 ttataatttt ttattggtaa acatatgcta aatccgcttc agtattttat tatgtttttt
     4921 aaaatgtgag aacttctgca ctacaaaatt cccttcacag agaagtataa tgtagttcca
     4981 acccgtgcta actacctttt ataaattcag tctagaaggt agtaatttct aatatttaga
     5041 tgtcttagta gagcgtatta tcatttaaag tgtattgtta gccttaagaa agcagctgat
     5101 agaagaactg aagtttctta ctcacgtggt ttaaaatgga gttcaaaaga ttgccattga
     5161 gttctgattg cagggactaa caatgttaat ctgataagga cagcaaaatc atcagaatca
     5221 gtgtttgtga ttgtgtttga atatgtggta acatatgaag gatatgacat gaagctttgt
     5281 atctcctttg gccttaagca agacctgtgt gctgtaagtg ccatttctca gtattttcaa
     5341 ggctctaacc cgccttcatc caatgtgtgg cctacaataa ctagcatttg ttgatttgtc
     5401 tcttgtatca aaattcccaa ataaaactta aaaccactga ctctgtcaga gaaactgaaa
     5461 cactgggaca tttcatcctt caattcctcg gtattgattt tatgttgatt gattttcaga
     5521 atttctctac agaaacgaaa gggaaatttt ctaatctgct ttatccatgt acttgcattt
     5581 cagacatgga catgctattg ttatttggct cataactgtt tccaaatgtt agttattatg
     5641 gacccaattt attaacaaca ttagctgatt tttacctatc agtattattt tatttctttt
     5701 agtttataga tctgtgcaac atttttgtac tgtatgtctt caaacctggc agtattaata
     5761 cccttcttac tgacatatgt acttttagtt ttagaaaact tttatattta tgtgtcttat
     5821 ttttatattt ctttatttat tacacagtgt agtgtataat actgtagttt gtattaatac
     5881 aataatatat tttagtatga aaatttggaa agttgataag atttaaagta gagatgcaat
     5941 tggttctcct gcattgagat ttgatttaac agtgttatgt taacatttat acttgccttg
     6001 gactgtagaa cagaacttaa atgggaatgt attagtttta caactacaat caagtcattt
     6061 tacctttacc cagtttttaa tataaaactt aaattttgaa attcactgtg tgactaatag
     6121 catgatgctc tgcagtttta ttaagaaatc agcctaacca tacaactctc atttccttag
     6181 taagccaaat taggattaac ttctataaac agtgttggga acaatgttta acattttgtg
     6241 ccaatttgtt cctgtattca tgtatgtaag ttacagatct gactcttcat ttttaagttc
     6301 cttgttacat catggtcatt ttctagtttt ttaccagact cccatctcac aataaaatgc
     6361 atcaacaagc ctgaactgct gtactaggaa ttcgatatca agcttatcga taatcaacct
     6421 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6481 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6541 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6601 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6661 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6721 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6781 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6841 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6901 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6961 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     7021 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7081 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7141 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7201 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7261 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     7321 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     7381 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     7441 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     7501 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     7561 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7621 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7681 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     7741 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7801 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7861 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7921 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7981 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8221 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8341 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8401 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8461 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8521 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8581 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8641 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8701 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8761 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8821 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8881 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8941 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     9001 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     9061 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     9121 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     9181 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     9241 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9301 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9361 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     9541 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9721 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9781 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11701 cccgaaaagt gccacctgac
//
