LOCUS       sequence-156351-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing SMAD4 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SMAD4_p.E134K
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg acaactttgt acaaaaaagt
       61 tggcaccatg gacaatatgt ctattacgaa tacaccaaca agtaatgatg cctgtctgag
      121 cattgtgcat agtttgatgt gccatagaca aggtggagag agtgaaacat ttgcaaaaag
      181 agcaattgaa agtttggtaa agaagctgaa ggagaaaaaa gatgaattgg attctttaat
      241 aacagctata actacaaatg gagctcatcc tagtaaatgt gttaccatac agagaacatt
      301 ggatgggagg cttcaggtgg ctggtcggaa aggatttcct catgtgatct atgcccgtct
      361 ctggaggtgg cctgatcttc acaaaaatga actaaaacat gttaaatatt gtcagtatgc
      421 gtttgactta aaatgtgata gtgtctgtgt gaatccatat cactacaaac gagttgtatc
      481 acctggaatt gatctctcag gattaacact gcagagtaat gctccatcaa gtatgatggt
      541 gaaggatgaa tatgtgcatg actttgaggg acagccatcg ttgtccactg aaggacattc
      601 aattcaaacc atccagcatc caccaagtaa tcgtgcatcg acagagacat acagcacccc
      661 agctctgtta gccccatctg agtctaatgc taccagcact gccaactttc ccaacattcc
      721 tgtggcttcc acaagtcagc ctgccagtat actggggggc agccatagtg aaggactgtt
      781 gcagatagca tcagggcctc agccaggaca gcagcagaat ggatttactg gtcagccagc
      841 tacttaccat cataacagca ctaccacctg gactggaagt aggactgcac catacacacc
      901 taatttgcct caccaccaaa acggccatct tcagcaccac ccgcctatgc cgccccatcc
      961 cggacattac tggcctgttc acaatgagct tgcattccag cctcccattt ccaatcatcc
     1021 tgctcctgag tattggtgtt ccattgctta ctttgaa
//
