LOCUS       sequence-156352-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A 
            variant) expression. Contains NsiI cloning site for U6-sgRNA 
            cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9n
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..124
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(157..222,223..260)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    429..563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    859..877
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    895..913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    931..949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    967..985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1003..1021
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1039..1057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gttctcctct tcctcatctc cgggcctttc gacctgcatc catctagatc tcgatcgagc
       61 agctgaagct tatggagaag ttactattcc gaagttccta ttctctagaa agtataggaa
      121 cttcgatccc cctcgatccc ccgggctgca gatctgtagg gcgcagtagt ccagggtttc
      181 cttgatgatg tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa
      241 ctcttcgcgg tctttccagt ggggatcgac ggtatcgata agcttgcaga tctgcgactc
      301 tagaggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
      361 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      421 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      541 atgtatctta tcatgtctgg atctgcgact ctagaggatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcgag tttaccatgc atgtgttcga
      841 ttctagattc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
      901 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
      961 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
     1021 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
     1081 tcagtgata
//
