LOCUS       sequence-156358-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A 
            variant) expression. Contains NsiI cloning site for U6-sgRNA 
            cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Dow LE, Fisher J, O'Rourke KP, Muley A, Kastenhuber ER, Livshits G, 
            Tschaharganeh DF, Socci ND, Lowe SW
  TITLE     Inducible in vivo genome editing with CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 
            2015 Feb 18.
  PUBMED    25690852
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3155"; 
            journalName: "Nat Biotechnol"; date: "2015-04"; volume: "33"; issue:
            "4"; pages: "390-4"
COMMENT     Sequence Label: c3GIC9n
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             214..234
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata atatggccct cgagtattga tcagtggatc cggtgccacc
      181 atgtacccat acgatgttcc agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg
      241 tccgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
      301 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      361 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      421 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      481 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      541 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
      601 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
      661 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
      721 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
      781 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
      841 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
      901 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
      961 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1021 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggaca
//
