LOCUS       sequence-156360-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter, Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-empty-pgk-hph
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   623..657
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(675..694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(676..694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(701..718)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(701..717)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
       61 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
      121 cgtccgaggg caaaggaata gtcgagaaat tgatgatcta ttaaacaata aagatgtcca
      181 catggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
      241 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc
      301 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatctataac
      361 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta
      421 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg
      481 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag
      541 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag
      601 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta
      661 aggtacccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
      721 gtc
//
