LOCUS       sequence-156365-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eIF4A1 Q195E mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Wolfe AL, Singh K, Zhong Y, Drewe P, Rajasekhar VK, Sanghvi VR, 
            Mavrakis KJ, Jiang M, Roderick JE, Van der Meulen J, Schatz JH, 
            Rodrigo CM, Zhao C, Rondou P, de Stanchina E, Teruya-Feldstein J, 
            Kelliher MA, Speleman F, Porco JA Jr, Pelletier J, Ratsch G, Wendel 
            HG
  TITLE     RNA G-quadruplexes cause eIF4A-dependent oncogene translation in 
            cancer.
  JOURNAL   Nature. 2014 Sep 4;513(7516):65-70. doi: 10.1038/nature13485. Epub 
            2014 Jul 27.
  PUBMED    25079319
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13485"; journalName: "Nature"; date: "2014-09-4- 4"; 
            volume: "513"; issue: "7516"; pages: "65-70"
COMMENT     Sequence Label: MIG-eIF4A1 Q195E
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      131..140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             143..169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaatta gatctcgatc ccgcgaaatt aatacgactc
       61 actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga
      121 gatatacata gccaccatgg cttacccata cgacgtccca gactacgcta tgtctgcgag
      181 tcaggattct cgatccagag acaatggccc cgacgggatg gagccggaag gcgtcatcga
      241 gagtaactgg aacgagattg tggatagctt tgatgacatg aatctctcag agtccctcct
      301 ccgtggtatt tatgcctatg gttttgagaa gccctctgcc atccagcagc gagctattct
      361 tccttgtatc aagggttatg atgtgattgc tcaagcccag tctgggactg ggaaaacagc
      421 tacatttgcc atatcaattc tgcagcagat tgaattagat ctaaaggcca ctcaggcttt
      481 ggttctggca cccacacgtg aattggctca gcagatacaa aaggtggtta tggcattagg
      541 agactacatg ggtgcctctt gtcatgcctg cattgggggc accaatgtgc gtgctgaggt
      601 gcagaagctg cagatggaag ctccccatat catcgtgggt acccctggcc gggtgtttga
      661 catgcttaac cggagatacc tgtcccccaa atacatcaag atgttcgtac tggatgaagc
      721 agatgaaatg ttaagccgag ggttcaagga tgagatctat gacatattcc agaagctcaa
      781 cagcaacaca caggtagttt tgttgtctgc tacaatgcct tctgatgtcc ttgaggtgac
      841 caagaaattt atgagagacc ctattcggat tcttgtcaag aaggaagaat tgaccctgga
      901 gggtatccgc caattctaca tcaatgtgga acgagaggag tggaagcttg acacattgtg
      961 tgact
//
