LOCUS       sequence-156374-         281 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330A-1x2
FEATURES             Location/Qualifiers
     source          1..281
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
       61 gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact
      121 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
      181 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
      241 taattatttt gtgcagcgat gggggcgggg gggggggggg g
//
