LOCUS       sequence-156381-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1_p.D44N
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcaccatg agcgacgtgg ctattgtgaa ggagggttgg ctgcacaaac gaggggagta
      121 catcaagacc tggcggccac gctacttcct cctcaagaat gatggcacct tcattggcta
      181 caaggagcgg ccgcagaatg tggaccaacg tgaggctccc ctcaacaact tctctgtggc
      241 gcagtgccag ctgatgaaga cggagcggcc ccggcccaac accttcatca tccgctgcct
      301 gcagtggacc actgtcatcg aacgcacctt ccatgtggag actcctgagg agcgggagga
      361 gtggacaacc gccatccaga ctgtggctga cggcctcaag aagcaggagg aggaggagat
      421 ggacttccgg tcgggctcac ccagtgacaa ctcaggggct gaagagatgg aggtgtccct
      481 ggccaagccc aagcaccgcg tgaccatgaa cgagtttgag tacctgaagc tgctgggcaa
      541 gggcactttc ggcaaggtga tcctggtgaa ggagaaggcc acaggccgct actacgccat
      601 gaagatcctc aagaaggaag tcatcgtggc caaggacgag gtggcccaca cactcaccga
      661 gaaccgcgtc ctgcagaact ccaggcaccc cttcctcaca gccctgaagt actctttcca
      721 gacccacgac cgcctctgct ttgtcatgga gtacgccaac gggggcgagc tgttcttcca
      781 cctgtcccgg gagcgtgtgt tctccgagga ccgggcccgc ttctatggcg ctgagattgt
      841 gtcagccctg gactacctgc actcggagaa gaacgtggtg taccgggacc tcaagctgga
      901 gaacctcatg ctggacaagg acgggcacat taagatcaca gacttcgggc tgtgcaagga
      961 ggggatcaag gacggtgcca ccatgaagac cttttgcggc acacctgagt acctggcccc
     1021 cgaggtgctg gaggacaatg actacggccg tgca
//
