LOCUS       sequence-156385-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminally truncated, mCherry-tagged EB1 as marker for 
            growing microtubule plus end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Ettinger A, van Haren J, Ribeiro SA, Wittmann T
  TITLE     Doublecortin Is Excluded from Growing Microtubule Ends and 
            Recognizes the GDP-Microtubule Lattice.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1549-55. doi: 
            10.1016/j.cub.2016.04.020. Epub 2016 May 26.
  PUBMED    27238282
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.020"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1549-55"
COMMENT     Sequence Label: pEB1deltaC-mCherry
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..841)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(409..428)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     678..696
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag
      121 atccggtgga tcccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
      181 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
      241 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
      301 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
      361 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
      421 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
      481 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
      541 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
      601 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
      661 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
      721 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
      781 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
      841 tgtcccgggt atactcttct tggcccttgc tcac
//
