LOCUS       sequence-156391-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knocking-in mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-mCherry
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..726
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(161..179)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     429..448
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(813..833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtcgacgccg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
       61 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      241 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      421 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      481 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      541 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      601 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      721 aagtaataga cgcgtgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      781 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      841 gctgctttaa tgccttt
//
