LOCUS       sequence-156394-       10958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knocking-in mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-mCherry
FEATURES             Location/Qualifiers
     source          1..10958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1822..2201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2203..2480
                     /label=chicken beta-actin promoter
     intron          2482..3499
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3423..3445
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3507..3526
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3569..4279
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3714..3732)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3982..4001
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4313..4901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4366..4386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4784..4795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4933..5409
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5132..5151)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5519..5538)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5584..5639
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5638..5657)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6000..6016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6024..6040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6048..6054,6055..6072,6073..6078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6093..6114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6188..7024
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7064..7083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7065..7083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7090..7107)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7090..7106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7121)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7244..7546
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7425..7444
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(7949..7968)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8563..8582
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(8943..9734,9735..9803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9566..9585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9927..10515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10416..10435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10669..10686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10803..10824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10839..10844,10845..10862,10863..10869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10877..10893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10901..10917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10938..10956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta
     1861 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1921 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1981 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2041 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2101 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2161 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     2221 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     2281 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg
     2341 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     2401 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     2461 agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg tgccccgctc
     2521 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     2581 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     2641 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     2701 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     2761 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     2821 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     2881 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     2941 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     3001 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     3061 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     3121 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     3181 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     3241 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     3301 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     3361 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     3421 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     3481 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     3541 tggcaaagaa ttcgtcgacg ccgccaccat ggtgagcaag ggcgaggagg ataacatggc
     3601 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3661 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3721 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3781 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     3841 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3901 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     3961 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4021 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     4081 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     4141 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     4201 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     4261 ggacgagctg tacaagtaat agacgcgtga attcgatatc aagcttatcg ataatcaacc
     4321 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4381 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4441 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4501 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4561 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4621 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4681 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt
     4741 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4801 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4861 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct
     4921 cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     4981 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     5041 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     5101 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     5161 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     5221 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     5281 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     5341 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     5401 cctgtccttc tgattttgta ggtaaccagg cgcgtgaatt cactcctcag gtgcaggctg
     5461 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     5521 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     5581 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     5641 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     5701 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     5761 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     5821 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     5881 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     5941 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     6001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     6121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cgacagtact
     6181 aagctttact agggacagga ttggtgacag aaaagcccca tccttaggcc tcctccttcc
     6241 tagtctcctg atattgggtc taacccccac ctcctgttag gcagattcct tatctggtga
     6301 cacaccccca tttcctggag ccatctctct ccttgccaga acctctaagg tttgcttacg
     6361 atggagccag agaggatcct gggagggaga gcttggcagg gggtgggagg gaaggggggg
     6421 atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta ccctctccca gaacctgagc
     6481 tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg tgctgcagct tccttacact
     6541 tcccaagagg agaagcagtt tggaaaaaca aaatcagaat aagttggtcc tgagttctaa
     6601 ctttggctct tcacctttct agtccccaat ttatattgtt cctccgtgcg tcagttttac
     6661 ctgtgagata aggccagtag ccagccccgt cctggcaggg ctgtggtgag gaggggggtg
     6721 tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc
     6781 cgcctctact ccctttctct ttctccatcc ttctttcctt aaagagtccc cagtgctatc
     6841 tgggacatat tcctccgccc agagcagggt cccgcttccc taaggccctg ctctgggctt
     6901 ctgggtttga gtccttggca agcccaggag aggcgctcag gcttccctgt cccccttcct
     6961 cgtccaccat ctcatgcccc tggctctcct gccccttccc tacaggggtt cctggctctg
     7021 ctctaagggc aagggcgaat tcgcggccgc taaattcaat tcgccctata gtgagtcgta
     7081 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7201 caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac
     7261 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     7321 gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     7381 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     7441 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     7501 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag
     7561 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
     7621 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     7681 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     7741 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     7801 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     7861 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7921 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7981 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8041 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     8101 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8161 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     8221 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     8281 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     8341 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     8401 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgc
     8461 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8521 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8581 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8641 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta
     8701 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8761 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8821 atacattcaa atatgtatcc gctcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8881 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9841 ggttattgtc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    10921 gattacgcca agctcagaat taaccctcac taaagggt
//
