LOCUS       sequence-156395-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-hM4Di-mCherry
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgccaccatg gccaacttca cacctgtcaa tggcagctcg ggcaatcagt ccgtgcgcct
       61 ggtcacgtca tcatcccaca atcgctatga gacggtggaa atggtcttca ttgccacagt
      121 gacaggctcc ctgagcctgg tgactgtcgt gggcaacatc ctggtgatgc tgtccatcaa
      181 ggtcaacagg cagctgcaga cagtcaacaa ctacttcctc ttcagcctgg cgtgtgctga
      241 tctcatcata ggcgccttct ccatgaacct ctacaccgtg tacatcatca agggctactg
      301 gcccctgggc gccgtggtct gcgacctgtg gctggccctg gactgcgtgg tgagcaacgc
      361 ctccgtcatg aaccttctca tcatcagctt tgaccgctac ttctgcgtca ccaagcctct
      421 cacctaccct gcccggcgca ccaccaagat ggcaggcctc atgattgctg ctgcctgggt
      481 actgtccttc gtgctctggg cgcctgccat cttgttctgg cagtttgtgg tgggtaagcg
      541 gacggtgccc gacaaccagt gcttcatcca gttcctgtcc aacccagcag tgacctttgg
      601 cacagccatt gctggcttct acctgcctgt ggtcatcatg acggtgctgt acatccacat
      661 ctccctggcc agtcgcagcc gagtccacaa gcaccggccc gagggcccga aggagaagaa
      721 agccaagacg ctggccttcc tcaagagccc actaatgaag cagagcgtca agaagccccc
      781 gcccggggag gccgcccggg aggagctgcg caatggcaag ctggaggagg cccccccgcc
      841 agcgctgcca ccgccaccgc g
//
