LOCUS       sequence-156396-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry 
            expression cassette driven by CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Chen Y, Xiong M, Dong Y, Haberman A, Cao J, Liu H, Zhou W, Zhang SC
  TITLE     Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse 
            Model of Parkinson's Disease.
  JOURNAL   Cell Stem Cell. 2016 Jun 2;18(6):817-26. doi: 
            10.1016/j.stem.2016.03.014. Epub 2016 Apr 28.
  PUBMED    27133795
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.03.014"; journalName: "Cell Stem Cell"; date: 
            "2016-06-2- 2"; volume: "18"; issue: "6"; pages: "817-26"
COMMENT     Sequence Label: AAVS1-Pur-CAG-hM4Di-mCherry
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..773)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(341..360)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     610..628
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcac gcgtggcgcg
       61 ccttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac
      121 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg
      181 taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg
      241 tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc
      301 tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      361 ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc
      421 agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc
      481 cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc
      541 acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg
      601 gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      661 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
      721 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catggtggcg
      781 accggtggat cccgcctggc agtgccgatg ttccgatact ggcacagcag caggtgccgg
      841 aaggtctttt taaaggtggc gttgcacaga gcata
//
