LOCUS       sequence-156409-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression copGFP and Puromycine, with an S/MAR element for episomal
            maintaince.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Jin C, Fotaki G, Ramachandran M, Nilsson B, Essand M, Yu D
  TITLE     Safe engineering of CAR T cells for adoptive cell therapy of cancer 
            using long-term episomal gene transfer.
  JOURNAL   EMBO Mol Med. 2016 May 6. pii: e201505869. doi: 
            10.15252/emmm.201505869.
  PUBMED    27189167
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Mol 
            Med. 2016 May 6. pii: e201505869. doi: 10.15252/emmm.201505869."
COMMENT     Sequence Label: pBMN(CMV-copGFP-Puro-SMAR)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..126)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(142..195)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cacgtctccc gccaacttga gaaggtcaaa attcaaagtc tgtttcaccg gtccggagcc
       61 attaattaag ggaccggggt tttcttccac gtctcctgct tgctttaaca gagagaagtt
      121 cgtggcacct gatccgctag cgggcccagg attctcctcg acgtcaccgc atgttagcag
      181 acttcctctg ccctccccgg agccctcgcg agatccggtg gagccgggtc cggcggtgcc
      241 gtccacggca gaattggacg actgagcgcg ggatctggcg aaggcgatgg gggtcttgaa
      301 ggcgtgctgg tactccacga tgcccagctc ggtgttgctg tgcagctcct ccacgcggcg
      361 gaaggcgaac atggggcccc cgttctgcag gatgctgggg tggatggcgc tcttgaagtg
      421 catgtggctg tccaccacga agctgtagta gccgccgtcg cgcaggctga aggtgcgggc
      481 gaagctgccc accagcacgt tatcgcccat ggggtgcagg tgctccacgg tggcgttgct
      541 gcggatgatc ttgtcggtga agatcacgct gtcctcgggg aagccggtgc ccaccacctt
      601 gaagtcgccg atcacgcggc cggcctcgta gcggtagctg aagctcacgt gcagcacgcc
      661 gccgtcctcg tacttctcga tgcgggtgtt ggtgtagccg ccgttgttga tggcgtgcag
      721 gaaggggttc tcgtagccgc tggggtaggt gccgaagtgg tagaagccgt agcccatcac
      781 gtggctcagc aggtaggggc tgaaggtcag ggcgcctttg gtgctcttca tcttgttggt
      841 catgcggccc tgcttggggg tgccctctcc gccgcccacc a
//
