LOCUS       sequence-156429-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HO endonuclease expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   McCusker J
  TITLE     McCusker Lab Unpublished Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHS3
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..34
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     266..285
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 attacgccaa gctcgaatta accctcacta aagggaacaa aagctggtac cgggcccccc
       61 ctcgaggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
      121 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
      181 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
      241 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      301 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccac gaaaatgatg
      361 tgaatgaata catgaaagat tcatgagatc tgacaacatg gtagacgtgt gtgtctcatg
      421 gaaattgatg cagttgaaga catgtgcgtc acgaaaaaag aaatcaatcc tacacagggc
      481 ttaagggcaa atgtattcat gtgtgtcacg aaaagtgatg taactaaata cacgattacc
      541 atggaaatta acgtaccttt tttgtgcgtg tattgaaata ttatgacata ttacagaaag
      601 ggttcgcaag tcctgtttct atgcctttct cttagtaatt cacgaaataa acctatggtt
      661 tacgaaatga tccacgaaaa tcatgttatt atttacatca acatatcgcg aaaattcatg
      721 tcatgtccac attaacatca ttgcagagca acaattcatt ttcatagaga aatttgctac
      781 tatcactcat tagtactacc attggtacct actactttga attgtactac cgctgggcgt
      841 tattaggtgt gaaaccacga aaagttcaac ataacttcga ataaagtcgc ggaagaaagt
      901 aaacagctat tgctactcaa atgaggtttg cagaagcttg ttgaagcatg atgaagcgtt
      961 tctaacgcac tatt
//
