LOCUS       sequence-156436-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of EPHB1. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: EPHB1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gatcccaacg aagctgtccg ggagtttgcc aaggagattg
      181 atgtatcttt tgtgaaaatt gaagaggtca tcggagcagg ggagtttgga gaagtgtaca
      241 aggggcgttt gaaactgcca ggcaagaggg aaatctacgt ggccatcaag accctgaagg
      301 cagggtactc ggagaagcag cgtcgggact ttctgagtga ggcgagcatc atgggccagt
      361 tcgaccatcc taacatcatt cgcctggagg gtgtggtcac caagagtcgg cctgtcatga
      421 tcatcacaga gttcatggag aatggtgcat tggattcttt cctcaggcaa aatgacgggc
      481 agttcaccgt gatccagctt gtgggtatgc tcaggggcat cgctgctggc atgaagtacc
      541 tggctgagat gaattatgtg catcgggacc tggctgctag gaacattctg gtcaacagta
      601 acctggtgtg caaggtgtcc gactttggcc tctcccgcta cctccaggat gacacctcag
      661 atcccaccta caccagctcc ttgggaggga agatccctgt gagatggaca gctccagagg
      721 ccatcgccta ccgcaagttc acttcagcca gcgacgtttg gagctatggg atcgtcatgt
      781 gggaagtcat gtcatttgga gagagaccct attgggatat gtccaaccaa gatgtcatca
      841 atgccatcga gcaggactac cggctgcc
//
