LOCUS       sequence-156442-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK08. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK08_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atgagcagaa gcaagcgtga caacaatttt tatagtgtag
      181 agattggaga ttctacattc acagtcctga aacgatatca gaatttaaaa cctataggct
      241 caggagctca aggaatagta tgcgcagctt atgatgccat tcttgaaaga aatgttgcaa
      301 tcaagaagct aagccgacca tttcagaatc agactcatgc caagcgggcc tacagagagc
      361 tagttcttat gaaatgtgtt aatcacaaaa atataattgg ccttttgaat gttttcacac
      421 cacagaaatc cctagaagaa tttcaagatg tttacatagt catggagctc atggatgcaa
      481 atctttgcca agtgattcag atggagctag atcatgaaag aatgtcctac cttctctatc
      541 agatgctgtg tggaatcaag caccttcatt ctgctggaat tattcatcgg gacttaaagc
      601 ccagtaatat agtagtaaaa tctgattgca ctttgaagat tcttgacttc ggtctggcca
      661 ggactgcagg aacgagtttt atgatgacgc cttatgtagt gactcgctac tacagagcac
      721 ccgaggtcat ccttggcatg ggctacaagg aaaacgtgga tttatggtct gtggggtgca
      781 ttatgggaga aatggtttgc cacaaaatcc tctttccagg aagggactat attgatcagt
      841 gga
//
