LOCUS       sequence-156444-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of EPHB3. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: EPHB3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gaccctaatg aggctgttcg ggagtttgcc aaggagatcg
      181 acgtgtcctg cgtcaagatc gaggaggtga tcggagctgg ggaatttggg gaagtgtgcc
      241 gtggtcgact gaaacagcct ggccgccgag aggtgtttgt ggccatcaag acgctgaagg
      301 tgggctacac cgagaggcag cggcgggact tcctaagcga ggcctccatc atgggtcagt
      361 ttgatcaccc caatataatc cggctcgagg gcgtggtcac caaaagtcgg ccagttatga
      421 tcctcactga gttcatggaa aactgcgccc tggactcctt cctccggctc aacgatgggc
      481 agttcacggt catccagctg gtgggcatgt tgcggggcat tgctgccggc atgaagtacc
      541 tgtccgagat gaactatgtg caccgcgacc tggctgctcg caacatcctt gtcaacagca
      601 acctggtctg caaagtctca gactttggcc tctcccgctt cctggaggat gacccctccg
      661 atcctaccta caccagttcc ctgggcggga agatccccat ccgctggact gccccagagg
      721 ccatagccta tcggaagttc acttctgcta gtgatgtctg gagctacgga attgtcatgt
      781 gggaggtcat gagctatgga gagcgaccct actgggacat gagcaaccag gatgtcatca
//
