LOCUS       sequence-156446-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of M3K5. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: M3K5_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca agaagcacag aggaaggaga ctgtgaaagt gacttgctgg
      181 agtatgacta tgaatatgat gaaaatggtg acagagtcgt tttaggaaaa ggcacttatg
      241 ggatagtcta cgcaggtcgg gacttgagca accaagtcag aattgctatt aaggaaatcc
      301 cagagagaga cagcagatac tctcagcccc tgcatgaaga aatagcattg cataaacacc
      361 tgaagcacaa aaatattgtc cagtatctgg gctctttcag tgagaatggt ttcattaaaa
      421 tcttcatgga gcaggtccct ggaggaagtc tttctgctct ccttcgttcc aaatggggtc
      481 cattaaaaga caatgagcaa acaattggct tttatacaaa gcaaatactg gaaggattaa
      541 aatatctcca tgacaatcag atagttcacc gggacataaa gggtgacaat gtgttgatta
      601 atacctacag tggtgttctc aagatctctg acttcggaac atcaaagagg cttgctggca
      661 taaacccctg tactgaaact tttactggta ccctccagta tatggcacca gaaataatag
      721 ataaaggacc aagaggctac ggaaaagcag cagacatctg gtctctgggc tgtacaatca
      781 ttgaaatggc cacaggaa
//
