LOCUS       sequence-156448-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of E2AK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: E2AK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gagacgaaat atacggtgga taaacgcttt ggtatggatt
      181 tcaaggagat tgaactgatc ggatcggggg ggtttggcca ggtatttaaa gcgaaacacc
      241 ggatcgacgg gaaaacttac gtaattaaac gcgtaaagta caacaatgaa aaagccgagc
      301 gggaagtgaa agctctggcg aaactggatc acgtgaatat tgtccattat aacggttgct
      361 gggatgggtt tgactatgat ccggaaacgt cggatgatag cctggagtct agtgattacg
      421 acccggaaaa ctcgaaaaac tcatcgcgct cgaaaacaaa atgcctgttt attcagatgg
      481 aattttgcga taaaggcacg ctggaacagt ggatcgaaaa acgccgcggg gaaaaactgg
      541 ataaagtcct ggcccttgaa ctgtttgaac agattacaaa aggggttgac tatatccata
      601 gtaaaaagtt aatccaccgt gacctgaaac ctagtaatat ctttttggta gacactaaac
      661 aggtaaagat tggtgacttc gggctggtca catccctgaa aaatgatggc aagcgtacgc
      721 gcagtaaagg taccctccgc tacatgtcgc cagagcagat ttcctcacaa gattatggta
      781 aagaagttga tctgtacgcg cttggactta tcctggcaga
//
