LOCUS       sequence-156450-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP-based BiFC vector for transient expression of CC (155-238) in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBCC155
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    26..81
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     295..316
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      380..632
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(670..686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(683..705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    694..710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(718..724,725..742,743..748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    763..784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(901..918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggacaggct tcttgagatc cttcaacaat taccaacaac aacaaacaac aaacaacatt
       61 acaattacta tttacaatta cagtcgactc tagaggatcc atggccgaca agcagaagaa
      121 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      181 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      241 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      301 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
      361 aagcggccgc ccggctgcag atcgttcaaa catttggcaa taaagtttct taagattgaa
      421 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
      481 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
      541 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
      601 atcgcgcgcg gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagaatt
      661 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      721 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      781 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      841 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      961 aaaggcggta atacggttat ccacagaatc aggggata
//
