LOCUS       sequence-156451-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CSK22. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CSK22_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             38..60
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             80..109
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             110..130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agcggccgcc ctgcaggact cgagttctag aaataatttt gtttaacttt aagaaggaga
       61 tatacatatg aaatcttctc accatcacca tcaccatcac catcaccatg aaaacctgta
      121 cttccaatcc aatgcaatgc ccggcccggc cgcgggcagc agggcccggg tctacgccga
      181 ggtgaacagt ctgaggagcc gcgagtactg ggactacgag gctcacgtcc cgagctgggg
      241 taatcaagat gattaccaac tggttcgaaa acttggtcgg ggaaaatata gtgaagtatt
      301 tgaggccatt aatatcacca acaatgagag agtggttgta aaaatcctga agccagtgaa
      361 gaaaaagaag ataaaacgag aggttaagat tctggagaac cttcgtggtg gaacaaatat
      421 cattaagctg attgacactg taaaggaccc cgtgtcaaag acaccagctt tggtatttga
      481 atatatcaat aatacagatt ttaagcaact ctaccagatc ctgacagact ttgatatccg
      541 gttttatatg tatgaactac ttaaagctct ggattactgc cacagcaagg gaatcatgca
      601 cagggatgtg aaacctcaca atgtcatgat agatcaccaa cagaaaaagc tgcgactgat
      661 agattggggt ctggcagaat tctatcatcc tgctcaggag tacaatgttc gtgtagcctc
      721 aaggtacttc a
//
