LOCUS       sequence-156455-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PAK7. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PAK7_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca tccagggtgt cccatgaaca gtttcgggcg gccctgcagc
      181 tggtggtcag cccaggagac cccagggaat acttggccaa ctttatcaaa atcggggaag
      241 gctcaaccgg catcgtatgc atcgccaccg agaaacacac agggaaacaa gttgcagtga
      301 agaaaatgga cctccggaag caacagagac gagaactgct tttcaatgag gtcgtgatca
      361 tgcgggatta ccaccatgac aatgtggttg acatgtacag cagctacctt gtcggcgatg
      421 agctctgggt ggtcatggag tttctagaag gtggtgcctt gacagacatt gtgactcaca
      481 ccagaatgaa tgaagaacag atagctactg tctgcctgtc agttctgaga gctctctcct
      541 accttcataa ccaaggagtg attcacaggg acataaaaag tgactccatc ctcctgacaa
      601 gcgatggccg gataaagttg tctgattttg gtttctgtgc tcaagtttcc aaagaggtgc
      661 cgaagaggaa atcattggtt ggcactccct actggatggc ccctgaggtg atttctaggc
      721 taccttatgg gacagaggtg gacatctggt ccctcgggat catggtgata gaaatgattg
      781 atggcgagcc cccctacttc aatgagcctc ccctccaggc gatgcggagg atccgg
//
