LOCUS       sequence-156459-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC4. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC4_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca tcttcggtca ccgccagtgc ggccccgggg accgcgagcc
      181 tcgtcccgga ttactggatc gacggctcca acagggatgc gctgagcgat ttcttcgagg
      241 tggagtcgga gctgggacgg ggtgctacat ccattgtgta cagatgcaaa cagaagggga
      301 cccagaagcc ttatgctctc aaagtgttaa agaaaacagt ggacaaaaaa atcgtaagaa
      361 ctgagatagg agttcttctt cgcctctcac atccaaacat tataaaactt aaagagatat
      421 ttgaaacccc tacagaaatc agtctggtcc tagaactcgt cacaggagga gaactgtttg
      481 ataggattgt ggaaaaggga tattacagtg agcgagatgc tgcagatgcc gttaaacaaa
      541 tcctggaggc agttgcttat ctacatgaaa atgggattgt ccatcgtgat ctcaaaccag
      601 agaatcttct ttatgcaact ccagccccag atgcaccact caaaatcgct gattttggac
      661 tctctaaaat tgtggaacat caagtgctca tgaagacagt atgtggaacc ccagggtact
      721 gcgcacctga aattcttaga ggttgtgcct atggacctga ggtggacatg tggtctgtag
      781 gaataatcac ctacatctta ctttgtggat ttgaaccatt ctatgatgaa agaggcgatc
      841 agttcatgtt caggagaat
//
