LOCUS       sequence-156462-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC2G. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC2G_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gccacctgca cacgtttcac cgacgactac cagctcttcg
      181 aggagcttgg caagggtgct ttctctgtgg tccgcaggtg tgtgaagaaa acccccacgc
      241 aggagtacgc agcaaaaatc atcaatacca agaaattgtc tgcccgggat caccagaaac
      301 tagaacgtga ggctcggata tgtcgacttc tgaaacatcc aaacatcgtg cgcctccatg
      361 acagtatttc tgaagaaggg tttcactacc tcgtgtttga ccttgttacc ggcggggagc
      421 tgtttgaaga cattgtggcc agagagtact acagtgaagc agatgccagc cactgtatac
      481 atcagattct ggagagtgtt aaccacatcc accagcatga catcgtccac agggacctga
      541 agcctgagaa cctgctgctg gcgagtaaat gcaagggtgc cgccgtcaag ctggctgatt
      601 ttggcctagc catcgaagta cagggagagc agcaggcttg gtttggtttt gctggcaccc
      661 caggttactt gtcccctgag gtcttgagga aagatcccta tggaaaacct gtggatatct
      721 gggcctgcgg ggtcatcctg tatatcctcc tggtgggcta tcctcccttc tgggatgagg
      781 atcagcacaa gctgtatcag cagatcaagg ctggagccta tgatttccca tcaccagaat
      841 gggacacggt aactc
//
