LOCUS       sequence-156464-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1G3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1G3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca ggagttttaa tggttggacc taactttaga gttggaaaaa
      181 aaattggatg tggcaatttt ggagaattac gattagggaa aaatttatac acaaatgaat
      241 atgtggcaat taagttggag cccatgaaat caagagcacc acagctacat ttggaataca
      301 gattctataa gcagttagga tctggagatg gtatacctca agtttactat ttcggccctt
      361 gtggtaaata caatgctatg gtgctggaac tgctgggacc tagtttggaa gacttgtttg
      421 acttgtgtga cagaacattt tctcttaaaa cagttctcat gatagctata caactgattt
      481 ctcgcatgga atatgtccat tcaaagaact tgatatacag agatgtaaaa cctgagaact
      541 tcttaatagg acgaccagga aacaaaaccc agcaagttat tcacattata gattttgctt
      601 tggcaaagga atatattgat ccggagacaa agaaacacat accatacaga gaacacaaga
      661 gccttacagg aacagctaga tatatgagca taaacacaca tttaggaaaa gaacaaagta
      721 gaagagacga tttagaagct ttaggtcata tgttcatgta ttttctgaga ggcagtcttc
      781 cttggcaagg cttaaaggct gacacattaa aggagaggta tcagaa
//
