LOCUS       sequence-156466-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MK11. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MK11_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca cgcgccggct tctaccggca ggagctgaac aagaccgtgt
      181 gggaggtgcc gcagcggctg caggggctgc gcccggtggg ctccggcgcc tacggctccg
      241 tctgttcggc ctacgacgcc cggctgcgcc agaaggtggc ggtgaagaag ctgtcgcgcc
      301 ccttccagtc gctgatccac gcgcgcagaa cgtaccggga gctgcggctg ctcaagcacc
      361 tgaagcacga gaacgtcatc gggcttctgg acgtcttcac gccggccacg tccatcgagg
      421 acttcagcga agtgtacttg gtgaccaccc tgatgggcgc cgacctgaac aacatcgtca
      481 agtgccaggc gctgagcgac gagcacgttc aattcctggt ttaccagctg ctgcgcgggc
      541 tgaagtacat ccactcggcc gggatcatcc accgggacct gaagcccagc aacgtggctg
      601 tgaacgagga ctgtgagctc aggatcctgg atttcgggct ggcgcgccag gcggacgagg
      661 agatgaccgg ctatgtggcc acgcgctggt accgggcacc tgagatcatg ctcaactgga
      721 tgcattacaa ccaaacagtg gatatctggt ccgtgggctg catcatggct gagctgctcc
      781 agg
//
