LOCUS       sequence-156468-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of NEK1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: NEK1_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca atggagaagt atgttagact acagaagatt ggagaaggtt
      181 catttggaaa agccattctt gttaaatcta cagaagatgg cagacagtat gttatcaagg
      241 aaattaacat ctcaagaatg tccagtaaag aaagagaaga atcaaggaga gaagttgcag
      301 tattggcaaa catgaagcat ccaaatattg tccagtatag agaatcattt gaagaaaatg
      361 gctctctcta catagtaatg gattactgtg agggagggga tctgtttaag cgaataaatg
      421 ctcagaaagg cgttttgttt caagaggatc agattttgga ctggtttgta cagatatgtt
      481 tggccctgaa acatgtacat gatagaaaaa ttcttcatcg agacattaaa tctcagaaca
      541 tatttttaac taaagatgga acagtacaac ttggagattt tggaattgct agagttctta
      601 atagtactgt agagctggct cgagcttgca tagggacccc atactacttg tcacctgaaa
      661 tctgtgaaaa caaaccttac aataataaaa gtgacatttg ggctctgggg tgtgtccttt
      721 atgagctgtg tacacttaaa catgcttttg a
//
