LOCUS       sequence-156470-       10751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myristoylated-FLAG-tagged Akt2, constitutively active.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
  TITLE     Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated
            GLUT4 exocytosis through cortical actin remodeling.
  JOURNAL   Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
  PUBMED    25575350
REFERENCE   2  (bases 1 to 10751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-01-9"; volume: "6:5951. doi"; pages: " 
            10.1038/ncomms6951"
COMMENT     Sequence Label: pLenti-FLAG-Akt2-CA
FEATURES             Location/Qualifiers
     source          1..10751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2004..3215
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3052..3071
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3269..3289
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3308..3331
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4825..5413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4878..4898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5296..5307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5416..5432)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5417..5433)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5938..6118
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6144..6161)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6150..6374
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6420..6848
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6507..6526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6717..6738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6857..6877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6862..7191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7042..7177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7104..7123
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7239..7286
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7305..7679
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7809..7930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7846..7865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7900..7919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7979..7995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7979..7995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7992..8014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8003..8019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8027..8033,8034..8051,8052..8057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8072..8093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8210..8227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8381..8969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8461..8480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9140..9931,9932..10000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9763..9782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10001..10105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10180..10199)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10371..10750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taacccgtgt cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     2041 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     2101 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     2161 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2221 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2281 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2341 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2401 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2461 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2521 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2581 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2641 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2701 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2761 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2821 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2881 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2941 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     3001 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     3061 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     3121 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3181 ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttgggctgca ggtcgactct
     3241 agaggatcga attcttcgaa gctaggccat gggttcctcc aaatccaagc ccaaggctag
     3301 cgccatggac tacaaggacg atgacgataa gggaattgat ccaatgaatg aggtgtctgt
     3361 catcaaagaa ggctggctcc acaagcgtgg tgaatacatc aagacctgga ggccacggta
     3421 cttcctgctg aagagcgacg gctccttcat tgggtacaag gagaggcccg aggcccctga
     3481 tcagactcta ccccccttaa acaacttctc cgtagcagaa tgccagctga tgaagaccga
     3541 gaggccgcga cccaacacct ttgtcatacg ctgcctgcag tggaccacag tcatcgagag
     3601 gaccttccac gtggattctc cagacgagag ggaggagtgg atgcgggcca tccagatggt
     3661 cgccaacagc ctcaagcagc gggccccagg cgaggacccc atggaccaca agtgtggctc
     3721 ccccagtgac tcctccacga ctgaggagat ggaagtggcg gtcagcaagg cacgggctaa
     3781 agtgaccatg aatgacttcg actatctcaa actccttggc aagggaacct ttggcaaagt
     3841 catcctggtg cgggagaagg ccactggccg ctactacgcc atgaagatcc tgcggaagga
     3901 agtcatcatt gccaaggatg aagtcgctca cacagtcacc gagagccggg tcctccagaa
     3961 caccaggcac ccgttcctca ctgcgctgaa gtatgccttc cagacccacg accgcctgtg
     4021 ctttgtgatg gagtatgcca acgggggtga gctgttcttc cacctgtccc gggagcgcgt
     4081 cttcacagag gagcgggccc ggttttatgg tgcagagatt gtctcggctc ttgagtactt
     4141 gcactcgcgg gacgtggtat accgcgacat caagctggaa aacctcatgc tggacaaaga
     4201 tggccacatc aagatcactg actttggcct ctgcaaagag ggcatcagtg acggggccac
     4261 catgaaaacc ttctgtggga ccccggagta cctggcgcct gaggtgctgg aggacaatga
     4321 ctatggccgg gccgtggact ggtgggggct gggtgtggtc atgtacgaga tgatgtgcgg
     4381 ccgcctgccc ttctacaacc aggaccacga gcgcctcttc gagctcatcc tcatggaaga
     4441 gatccgcttc ccgcgcacgc tcagccccga ggccaagtcc ctgcttgctg ggctgcttaa
     4501 gaaggacccc aagcagaggc ttggtggggg gcccagcgat gccaaggagg tcatggagca
     4561 caggttcttc ctcagcatca actggcagga cgtggtccag aagaagctcc tgccaccctt
     4621 caaacctcag gtcacgtccg aggtcgacac aaggtacttc gatgatgaat ttaccgccca
     4681 gtccatcaca atcacacccc ctgaccgcta tgacagcctg ggcttactgg agctggacca
     4741 gcggacccac ttcccccagt tctcctactc ggccagcatc cgcgagtgag aattcgttaa
     4801 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4861 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4921 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4981 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5041 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5101 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5161 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5221 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5281 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5341 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5401 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     5461 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     5521 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     5581 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5641 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     5701 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     5761 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     5821 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     5881 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     5941 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6001 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6061 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     6121 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     6181 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     6241 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     6301 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     6361 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6421 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6481 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6541 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6601 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6661 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6721 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6781 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6841 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7021 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7081 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     7141 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7201 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     7261 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     7321 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     7381 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     7441 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     7501 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     7561 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     7621 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     7681 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7741 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7801 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7921 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10141 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    10201 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    10261 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    10321 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
    10381 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    10441 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10501 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10561 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10621 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10681 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10741 tattaccatg g
//
