LOCUS       sequence-156473-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KS6A1. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KS6A1_HUMAN_D1
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             84..113
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             114..134
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgaaatct tctcaccatc accatcacca tcaccatcac catgaaaacc
      121 tgtacttcca atccaatgca gttttttccg acggatacgt tgtcaaagag acaattggtg
      181 tgggtagtta ttctgagtgc aaacgctgcg ttcacaaagc gacaaacatg gaatatgccg
      241 ttaaagtgat cgataaaagc aaacgcgatc cgagtgaaga aattgagatt ctgctgcgct
      301 acggacagca tccgaacatc attaccctga aagatgtgta tgatgatgga aaacatgtgt
      361 atcttgtcac agagttaatg cgtggtggtg aattactgga taaaattctg cgtcagaaat
      421 tcttttcaga acgggaggcg agttttgttt tgcatacaat tggcaaaact gtagagtatc
      481 ttcactcgca gggtgtcgtg catcgtgatc tgaagccgag taatatcctg tacgtggatg
      541 agagcggcaa tccggaatgc cttcgcatct gcgattttgg atttgccaaa cagctgcgtg
      601 cggaaaatgg cttactgatg acgccctgct acaccgctaa ttttgtggca ccagaagtac
      661 tcaaacgtca gggctatgat gagggctgcg acatttggtc tctgggtatc ctgctgtaca
      721 cgatgctggc gggttacact ccgtttgcta atggtcccag tgatacgcct gaggaaatcc
      781 tcacgcgcat tggctc
//
