LOCUS       sequence-156497-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CFP-based BiFC vector for transient expression of CC (155-238) in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBCC155
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..62)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    276..331
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(351..696)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(388..408)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(756..773)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(756..772)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(765..787)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
       61 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
      121 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      181 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttggccttg atgccgttct
      241 tctgcttgtc ggccatggat cctctagagt cgactgtaat tgtaaatagt aattgtaatg
      301 ttgtttgttg tttgttgttg ttggtaattg ttgaaggatc tcaagaagcc tgtcctctcc
      361 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
      421 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
      481 acgtcttctt tttccacgat gttcctcgtg ggtgggggtc catctttggg accactgtcg
      541 gtagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggagcca
      601 ccttcctttt ctactgtcct ttcgatgaag tgacagatag ctgggcaatg gaatccgagg
      661 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctctggtc ttctgagggg
      721 ggatcctcta gagcctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt
      781 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      841 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      901 aatggcgaat ggcgcctgat gcggtattt
//
