LOCUS       sequence-156527-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing THEMIS , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_THEMIS_p.R196I
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcaccatg gcattatcac tggaagaatt cgtccactcc cttgacctca ggaccctacc
      121 cagggttcta gaaatccagg caggcatcta tcttgaaggc tctatttatg aaatgtttgg
      181 aaatgaatgc tgtttttcaa caggagaagt gattaaaatt actggtctca aagttaagaa
      241 gatcatagct gaaatttgtg agcagattga aggttgtgag tctctacagc catttgaact
      301 gcctatgaat tttccaggtc tttttaagat tgtggctgat aaaactccat accttactat
      361 ggaagaaatc acaaggacca ttcatattgg accaagtaga ctagggcatc cttgcttcta
      421 tcatcagaag gatataaaac tagagaacct catcataaag cagggtgagc aaatcatgct
      481 caactcagtt gaagagattg atggagaaat aatggtgagc tgtgcagtag caaggaatca
      541 tcaaactcac tcatttaatt tgcctttgtc acaagaagga gaattctacg agtgtgaaga
      601 tgaacgtatt tacactctaa aggagattgt tgaatggaag attcctaaga acataacaag
      661 aactgtaaac cttacagatt tttcaaataa gtgggactca acgaatccat ttcctaaaga
      721 cttttatggt accctgattc tcaagcctgt ttatgaaatt caaggtgtga tgaaatttcg
      781 aaaagatata atccgcatcc tccccagtct agatgtcgaa gtcaaagaca tcactgattc
      841 ttacgatgct aactggtttc ttcagctgtt atcaacagaa gatctttttg aaatgactag
      901 taaagagttc cccatagtga ctgaagtcat agaagcacct gaaggaaacc acctgcccca
      961 aagcatttta cagcctggga aaaccattgt gatcca
//
