LOCUS       sequence-156530-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker
            E
  TITLE     PEAR, a flexible fluorescent reporter for the identification and 
            enrichment of successfully prime edited cells.
  JOURNAL   Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504.
  PUBMED    35196219
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-23"; volume: "11. pii: 69504. doi"; pages: " 
            10.7554/eLife.69504"
COMMENT     Sequence Label: pX330-Flag-wtSpCas9-H840A
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..100
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             107..127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcctttttt tcaggttgga ccggtgccac catggactat aaggaccacg acggagacta
       61 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
      121 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
      181 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa
      241 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
      301 gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg ccagaagaag
      361 atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca acgagatggc
      421 caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg aagaggataa
      481 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
      541 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
      601 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcga
      661 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgc
//
