LOCUS       sequence-156531-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker
            E
  TITLE     PEAR, a flexible fluorescent reporter for the identification and 
            enrichment of successfully prime edited cells.
  JOURNAL   Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504.
  PUBMED    35196219
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-23"; volume: "11. pii: 69504. doi"; pages: " 
            10.7554/eLife.69504"
COMMENT     Sequence Label: pX330-Flag-wtSpCas9-H840A
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat
       61 cagcttggcg ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga
      121 gggcacgttg tcgctcttgc cccggttctt gtcgcttctg gtcagcacct tgttgtcgat
      181 ggagtcgtcc ttcagaaagc tctgaggcac gatggcgtcc acatcgtagt cggacagccg
      241 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
      301 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
      361 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
      421 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
      481 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
      541 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
      601 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
      661 tctgttggcg aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc
      721 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
      781 cagctgcttc atcactttgt cgtcga
//
