LOCUS       sequence-156535-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  enhanced SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  TITLE     crispr/cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX330-Flag-eSpCas9-H840A
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             93..113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gttggaccgg tgccaccatg gactataagg accacgacgg agactacaag gatcatgata
       61 ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc
      121 acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg
      181 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
      241 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
      301 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
      361 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
      421 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
      481 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
      541 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
      601 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
//
