LOCUS       sequence-156537-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  high fidelity SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  TITLE     crispr/cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX330-Flag-HFSpCas9-H840A
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..87
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             94..114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggttggaccg gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat
       61 attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc
      121 cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct
      181 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
      241 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
      301 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
      361 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
      421 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
      481 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
      541 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
      601 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
      661 cccgacaaca gcgacgtgga caagctgttc atc
//
