LOCUS       sequence-156542-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector with mCherry transfection control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor 
            E, Welker E
  TITLE     Crossing enhanced and high fidelity SpCas9 nucleases to optimize 
            specificity and cleavage.
  JOURNAL   Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
  PUBMED    28985763
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2017-10-6"; volume: "18(1):190. doi"; pages: " 
            10.1186/s13059-017-1318-8"
COMMENT     Sequence Label: pmCherry_gRNA
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(177..195)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcactttttt tcaggttgga ccggtgccac catggtgagc aagggcgagg aggataacat
       61 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      121 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      181 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      241 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      301 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      361 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      421 cggcaccaac ttcccctccg acggccccgt aatgcagaaa aagaccatgg gctgggaggc
      481 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
      541 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      601 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      661 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      721 catggacgag ctgtacaagt aggatccacc ggatctagat aactgatcat aatcagccat
      781 accacatttg tagaggtttt acttgcttta aa
//
