LOCUS       sequence-156545-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses eGFP along with an U6 promoter driven gRNA which cleaves 
            the plasmid in-cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, 
            Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, 
            Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E
  TITLE     A convenient method to pre-screen candidate guide RNAs for 
            CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 
            'self-cleaving' GFP-expression plasmid.
  JOURNAL   DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029.
  PUBMED    28679166
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2017 Jun 30. doi: 10.1093/dnares/dsx029."
COMMENT     Sequence Label: pSc1
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        47..122
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atatcttgtg gaaaggacga aacaccggcg caacgcgatc gcgtaagttt tagagctaga
       61 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      121 gcttttttcc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
      181 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
      241 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
      301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      781 aactgagata cctacagcgt gagctatga
//
