LOCUS       sequence-156557-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA 
            which cleaves the plasmid in-cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Talas A, Kulcsar PI, Weinhardt N, Borsy A, Toth E, Szebenyi K, 
            Krausz SL, Huszar K, Vida I, Sturm A, Gordos B, Hoffmann OI, 
            Bencsura P, Nyeste A, Ligeti Z, Fodor E, Welker E
  TITLE     A convenient method to pre-screen candidate guide RNAs for 
            CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 
            'self-cleaving' GFP-expression plasmid.
  JOURNAL   DNA Res. 2017 Jun 30. doi: 10.1093/dnares/dsx029.
  PUBMED    28679166
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2017 Jun 30. doi: 10.1093/dnares/dsx029."
COMMENT     Sequence Label: pSc1-DD
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        34..109
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aggacgaaac accggcgcaa cgcgatcgcg taagttttag agctagaaat agcaagttaa
       61 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttccacc
      121 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
      181 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
      241 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      301 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      361 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      421 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      481 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      541 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      601 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      661 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      721 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      781 a
//
