LOCUS       sequence-156561-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) 
            in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Kodama Y, Wada M
  TITLE     Simultaneous visualization of two protein complexes in a single 
            plant cell using multicolor fluorescence complementation analysis.
  JOURNAL   Plant Mol Biol. 2009 May;70(1-2):211-7. doi: 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14.
  PUBMED    19219406
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2009-05"; volume: "70(1-2):211-7. doi"; pages: " 
            10.1007/s11103-009-9467-0. Epub 2009 Feb 14"
COMMENT     Sequence Label: pBGN155
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..152
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     392..413
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    478..533
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(555..601)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(590..610)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
       61 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      121 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
      181 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      241 ctgaagcact gcacgccgta ggtgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      301 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      361 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      421 accaccccgg tgaacagctc ctcgcccttg ctcaccatgg atcctctaga gtcgactgta
      481 attgtaaata gtaattgtaa tgttgtttgt tgtttgttgt tgttggtaat tgttgaagga
      541 tctcaagaag cctgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
      601 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
      661 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg
      721 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca
      781 atgatggcat ttgtaggagc caccttcctt ttctactgtc ctttcgatga agtgacagat
      841 agctgggcaa tggaatccga
//
