LOCUS       sequence-156569-       13296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the mTagtandem tomato gene to the GAPDH locus of 
            human cells. It is expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13296)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 13296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mtagtdTom_IRESMuro
FEATURES             Location/Qualifiers
     source          1..13296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5635..7065
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(7143..7717,7718..7720,7721..7729)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7310..7327)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7537..7556
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(12652..12671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12653..12671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12681..12698)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12681..12697)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12690..12712)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12838..13293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12975..12996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13187..13206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccgatggtg
     5641 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     5701 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5761 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     5821 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     5881 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5941 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     6001 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     6061 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     6121 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     6181 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     6241 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     6301 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     6361 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     6421 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6481 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6541 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     6601 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6661 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     6721 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     6781 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     6841 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     6901 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     6961 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     7021 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagaatt cgatatcaag
     7081 cttcaagtcg acggatccgc ccctctccct cccccccccc taatgacaag tcgacggatc
     7141 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     7201 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7261 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7321 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7381 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7441 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7501 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7561 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7621 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7681 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggcgcgtat
     7741 ggcaagactg accgaataca agcctaccgt gagactggca acaagagatg atgtcccaag
     7801 agccgtgcga accctggcag cagcattcgc agactaccca gccacaagac acactgtgga
     7861 ccccgatagg catatcgaga gagtgaccga gctgcaggaa ctgtttctga cacgggtggg
     7921 cctggatatt ggaaaggtct gggtggcaga tgatggggct gctgtggccg tctggacaac
     7981 cccagagtct gtggaagctg gagctgtctt cgctgagatc ggccctcgaa tggcagaact
     8041 gtctggctcc aggctggcag cacagcagca gatggaggga ctgctggcac cacacagacc
     8101 taaggaacca gcttggttcc tggcaactgt gggggtctct cccgaccatc agggcaaagg
     8161 actggggagt gccgtggtcc tgcctggagt ggaggctgca gaaagagctg gagtcccagc
     8221 ttttctggag acatctgccc cccggaacct gcctttctat gaacgcctgg gctttactgt
     8281 gaccgctgac gtggaggtgc ccgaaggccc ccgaacttgg tgcatgaccc gaaagccagg
     8341 agcataaatc tagaggcgcg ccatcgataa gcttagatcg agcacaagag gaagagagag
     8401 accctcactg ctggggagtc cctgccacac tcagtccccc accacactga atctcccctc
     8461 ctcacagttg ccatgtagac cccttgaaga ggggaggggc ctagggagcc gcaccttgtc
     8521 atgtaccatc aataaagtac cctgtgctca accagttact tgtcctgtct tattctaggg
     8581 tctggggcag aggggaggga agctgggctt gtgtcaaggt gagacattct tgctggggag
     8641 ggacctggta tgttctcctc agactgaggg tagggcctcc aaacagcctt gcttgcttcg
     8701 agaaccattt gcttcccgct cagacgtctt gagtgctaca ggaagctggc accactactt
     8761 cagagaacaa ggccttttcc tctcctcgct ccagtcctag gctatctgct gttggccaaa
     8821 catggaagaa gctattctgt gggcagcccc agggaggctg acaggtggag gaagtcaggg
     8881 ctcgcactgg gctctgacgc tgactggtta gtggagctca gcctggagct gagctgcagc
     8941 gggcaattcc agcttggcct ccgcagctgt gaggtcttga gcacgtgctc tattgctttc
     9001 tgtgccctcg tgtcttatct gaggacatcg tggccagccc ctaaggtctt caagcaggat
     9061 tcatctaggt aaaccaagta cctaaaacca tgcccaaggc ggtaaggact atataatgtt
     9121 taaaaatcgg taaaaatgcc cacctcgcat agttttgagg aagatgaact gagatgtgtc
     9181 agggtgactt atttccatca tcgtccttag gggaacttgg gtaggggcaa ggcgtgtagc
     9241 tgggacctag gtccagaccc ctggctctgc cactgaacgg ctcagttgct ttgggcagtt
     9301 actcccgggc ctcactttgc acgtgtgctt acctagtgga gacaaaagta catacctcgg
     9361 tagagcgcgc acgcctgtaa ccccagcact ttgggaggcc aaggtgggtg tatcacctga
     9421 ggtcaggagt ttgagaccag cctggccaac atggtgaaac tccgtctcta ctaaaattac
     9481 aaaaatcagc caggcttcat ggcacatgcc tatagtccca gctacaggca tgctgaagca
     9541 ggagaatcgc ttgcaccccg gaggcagagg ctgcagtgag ctgagaccac accactgcac
     9601 tccagcctag gcaacagagt atgagactcc atctcaaaaa aaaaaaaagt acctacctca
     9661 gagttcaaac tagtgaatat taggaagtgc ttgagacagt gacaccaaag tgcacaataa
     9721 atactcgcca gtttcattat tattaaagaa tccatttgaa tgtcagctca acacagcctc
     9781 ctataccgag gcattgtgaa ccgcatctcc ccagcttctc caggcttttc caagaatcag
     9841 ggacactgta gcctgttggt ctcagtgtat gacagacacg gaggaagcac atctttagct
     9901 gatacttaaa cagagaccct gagcgcacat acacccgcgc acacatgcat ggagcttcac
     9961 cttctctgtc attctgcagt gaccaggaga gcaagagctc ccacctccct tcaaaacact
    10021 gtgcccatcc cgggcactaa ggcctcttta aagcacggca cctccacgag ggagggccac
    10081 agccacatac actccacctg gcaggtggac agcgtgagca cgtggaccat agcagggaca
    10141 aggtgccccg gccagcccca acgccctctg ccgctgacag ggacagaagc cctctccagc
    10201 tgcgtgtgct gcagaggcca tgcgtagcct ccagctgcat tctattccac tccagtgcct
    10261 gggccagtta gcaccagtgt ggaagacagt gagctggctc cggacaacag ggatggagga
    10321 aaggtcccac attcacattc ctgatacgtg gacaaggtga ggggccgcaa tcgctctggc
    10381 agcattttaa agatggggaa gtagcagaca cccacgcgtg aaggcaggag agccccaact
    10441 gtggtggaaa tggccccaga atggtagggc caagcctagc tccagacacc ccagagccct
    10501 ggagaagcca agactgaggg agaaagcctg agggaggagc gccccagtcc ccagggaccg
    10561 gcctggtgca gagctgcagc tgatgttccc ctctgtgcag ccccaccctc tgcctcgctg
    10621 agctccctgc tgcgagggcc tcgggtgcaa gggggaggca ggtctctatc tcatggagct
    10681 gtcagatgag acatcgcgat cggagtcctc agcctcgctt ggcggcggcg gcgggtcgct
    10741 aagcgggacc gcagtgaaag caggagactt tctagaaaaa aacaccagtt gtcaaccttg
    10801 gggcaggcag gaatcctgaa gacggacggc actcctcctc ctgctgcctc accctctggc
    10861 agcccgtgag aagtaccgga agcgagggcg gggccgcggg atggcgaggg agcggcaggg
    10921 actgaactct ctccaaaccc accctgacag ggaaatgggc cccgcctgtg tcttgggaac
    10981 tcagaggctg aggtcaggca tgatggctca cgcctgtaat tccagcactt tgggaggcag
    11041 aggcgggtgg atcacgacgt caggagttca aggcaagcct ggccaacatg gtgaaacccc
    11101 atctctacta aaaatgcaaa aattagccag gtgtggtggc gggcgccttg gaggctgagg
    11161 caagataatc acttgaacct gggaggcgga ggttgtagtg agccaaaaaa aaaaaaaaaa
    11221 gaaatagctg aagtcacagt aggagagaag ctgctgagcc tccagcaccc tgactctagg
    11281 gccttggctt tatgtctatc tgcagtattt ttgtgatttt taaaaattca ctttcttgtt
    11341 gcggtgtaac ttacacaggg tcaaatgcac aaatcatggc cctgacttta gataaaaatc
    11401 tgcccccaca acccttctgt tccttgccag tttttaaact gcctctaacc aggggaacca
    11461 ccagagctgg tggccttggg aggtttcagc cctcccgtca tgaatggaca tagctcatcc
    11521 aactgccaag ggagagagct gtgggtctgg gccagcccca ccaggtaact cccaaagggc
    11581 agccccacag caagatgtga cccagtcatt gcctgagggt ctctggggct gtgttccaac
    11641 ctttctcccc gctgtgtccc cctggaaggc cccatgccca ggggaggcgc ctaccggtct
    11701 ccagactggg tgatgagccg gcggcaggtc tccatctgca cgtccaggcc gcgcttcatg
    11761 ctgcacatct ccatgtactc gtgcaggtgc cggttcatgt cgttcttggc cgtggccaac
    11821 tccagctgtg gtggggcagg cagggccatc ggggttaaca ggtggcgttc acagcgcctc
    11881 tgttgccccc gccaggaggc caacacgcca agagcagtgg ctgggccggg ggcccaggca
    11941 gccattactg aaggctcaga ttttaaaata aaccagacca aggcagaagg cagcgaatac
    12001 accattgttt cctctgcttt tcctggaaat ctttgcctat ggaggaatgc aaaggtactg
    12061 ctgcaggctg tgttctttta agactgataa gagtggtgag gaaagtcagt gtgggcggga
    12121 tggagcagat ccacaggctc ccagggccac acaggttcct atggggcccg gctcacccct
    12181 catcccctgt actgcgctgg tcctgggctg tcccctcact gcactgtgag ctcccgtaag
    12241 ggttagcgac aggttttcct catcctggca gacccagacc tcgaagggaa ttaactctct
    12301 ctgtgacccg tggcatacaa gcctggctaa ctgctgaaag gggcccaatc ctcttaccat
    12361 tctctcctta tcactttatt ttctcaccta aaagccattt tcagacacta aaaggaagac
    12421 tcccatattg gaggtgccat tgattctgag acctgtcctg atttcagaaa tgtcacacgt
    12481 ggaaaacacg gggctcagaa tcaaagaaat ccggtctccc tccccgggcc cattctgcag
    12541 cccacactct gaggcaggcg gagaggcagg gcccaggcag cacaaactca gtgccaggga
    12601 cagtcgatta attaagcgat cgcaggccgc ccgcggtgga gctccaattc gccctatagt
    12661 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    12721 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    12781 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa
    12841 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    12901 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    12961 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    13021 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    13081 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    13141 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    13201 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    13261 ccgccgcgct taatgcgccg ctacagggcg cgtcag
//
