LOCUS       sequence-156570-       12270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the Clover gene to the GAPDH locus of human cells. 
            Clover is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Clover-Muro
FEATURES             Location/Qualifiers
     source          1..12270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5635..5637,5638..5640,5641..6351)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5680..5701)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6288..6309
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6373..6426
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7051..7068)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7057..7281
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11626..11645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11627..11645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11655..11672)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11655..11671)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11664..11686)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(11812..12267)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11949..11970)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12161..12180
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccgatggtg
     5641 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5701 gtaaacggcc acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag
     5761 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5821 accaccttcg gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac
     5881 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag
     5941 gacgacggta cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6001 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6061 gagtacaact tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc
     6121 aaggctaact tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac
     6181 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     6241 agccatcagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     6301 gagttcgtga ccgccgccgg gattacacat ggcatggacg agctgtacaa ggaagggggc
     6361 ggctctggcg gggagggaag aggaagtctt ctaacatgcg gtgacgtgga ggaaaacccc
     6421 gggcccatcg aaatggcaag actgaccgaa tacaagccta ccgtgagact ggcaacaaga
     6481 gatgatgtcc caagagccgt gcgaaccctg gcagcagcat tcgcagacta cccagccaca
     6541 agacacactg tggaccccga taggcatatc gagagagtga ccgagctgca ggaactgttt
     6601 ctgacacggg tgggcctgga tattggaaag gtctgggtgg cagatgatgg ggctgctgtg
     6661 gccgtctgga caaccccaga gtctgtggaa gctggagctg tcttcgctga gatcggccct
     6721 cgaatggcag aactgtctgg ctccaggctg gcagcacagc agcagatgga gggactgctg
     6781 gcaccacaca gacctaagga accagcttgg ttcctggcaa ctgtgggggt ctctcccgac
     6841 catcagggca aaggactggg gagtgccgtg gtcctgcctg gagtggaggc tgcagaaaga
     6901 gctggagtcc cagcttttct ggagacatct gccccccgga acctgccttt ctatgaacgc
     6961 ctgggcttta ctgtgaccgc tgacgtggag gtgcccgaag gcccccgaac ttggtgcatg
     7021 acccgaaagc caggagcata acctgatcag cctcgactgt gccttctagt tgccagccat
     7081 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7141 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7201 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7261 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgatggcgc
     7321 gttcctcgag agatctatcg ataagcttag atcgagcaca agaggaagag agagaccctc
     7381 actgctgggg agtccctgcc acactcagtc ccccaccaca ctgaatctcc cctcctcaca
     7441 gttgccatgt agaccccttg aagaggggag gggcctaggg agccgcacct tgtcatgtac
     7501 catcaataaa gtaccctgtg ctcaaccagt tacttgtcct gtcttattct agggtctggg
     7561 gcagagggga gggaagctgg gcttgtgtca aggtgagaca ttcttgctgg ggagggacct
     7621 ggtatgttct cctcagactg agggtagggc ctccaaacag ccttgcttgc ttcgagaacc
     7681 atttgcttcc cgctcagacg tcttgagtgc tacaggaagc tggcaccact acttcagaga
     7741 acaaggcctt ttcctctcct cgctccagtc ctaggctatc tgctgttggc caaacatgga
     7801 agaagctatt ctgtgggcag ccccagggag gctgacaggt ggaggaagtc agggctcgca
     7861 ctgggctctg acgctgactg gttagtggag ctcagcctgg agctgagctg cagcgggcaa
     7921 ttccagcttg gcctccgcag ctgtgaggtc ttgagcacgt gctctattgc tttctgtgcc
     7981 ctcgtgtctt atctgaggac atcgtggcca gcccctaagg tcttcaagca ggattcatct
     8041 aggtaaacca agtacctaaa accatgccca aggcggtaag gactatataa tgtttaaaaa
     8101 tcggtaaaaa tgcccacctc gcatagtttt gaggaagatg aactgagatg tgtcagggtg
     8161 acttatttcc atcatcgtcc ttaggggaac ttgggtaggg gcaaggcgtg tagctgggac
     8221 ctaggtccag acccctggct ctgccactga acggctcagt tgctttgggc agttactccc
     8281 gggcctcact ttgcacgtgt gcttacctag tggagacaaa agtacatacc tcggtagagc
     8341 gcgcacgcct gtaaccccag cactttggga ggccaaggtg ggtgtatcac ctgaggtcag
     8401 gagtttgaga ccagcctggc caacatggtg aaactccgtc tctactaaaa ttacaaaaat
     8461 cagccaggct tcatggcaca tgcctatagt cccagctaca ggcatgctga agcaggagaa
     8521 tcgcttgcac cccggaggca gaggctgcag tgagctgaga ccacaccact gcactccagc
     8581 ctaggcaaca gagtatgaga ctccatctca aaaaaaaaaa aagtacctac ctcagagttc
     8641 aaactagtga atattaggaa gtgcttgaga cagtgacacc aaagtgcaca ataaatactc
     8701 gccagtttca ttattattaa agaatccatt tgaatgtcag ctcaacacag cctcctatac
     8761 cgaggcattg tgaaccgcat ctccccagct tctccaggct tttccaagaa tcagggacac
     8821 tgtagcctgt tggtctcagt gtatgacaga cacggaggaa gcacatcttt agctgatact
     8881 taaacagaga ccctgagcgc acatacaccc gcgcacacat gcatggagct tcaccttctc
     8941 tgtcattctg cagtgaccag gagagcaaga gctcccacct cccttcaaaa cactgtgccc
     9001 atcccgggca ctaaggcctc tttaaagcac ggcacctcca cgagggaggg ccacagccac
     9061 atacactcca cctggcaggt ggacagcgtg agcacgtgga ccatagcagg gacaaggtgc
     9121 cccggccagc cccaacgccc tctgccgctg acagggacag aagccctctc cagctgcgtg
     9181 tgctgcagag gccatgcgta gcctccagct gcattctatt ccactccagt gcctgggcca
     9241 gttagcacca gtgtggaaga cagtgagctg gctccggaca acagggatgg aggaaaggtc
     9301 ccacattcac attcctgata cgtggacaag gtgaggggcc gcaatcgctc tggcagcatt
     9361 ttaaagatgg ggaagtagca gacacccacg cgtgaaggca ggagagcccc aactgtggtg
     9421 gaaatggccc cagaatggta gggccaagcc tagctccaga caccccagag ccctggagaa
     9481 gccaagactg agggagaaag cctgagggag gagcgcccca gtccccaggg accggcctgg
     9541 tgcagagctg cagctgatgt tcccctctgt gcagccccac cctctgcctc gctgagctcc
     9601 ctgctgcgag ggcctcgggt gcaaggggga ggcaggtctc tatctcatgg agctgtcaga
     9661 tgagacatcg cgatcggagt cctcagcctc gcttggcggc ggcggcgggt cgctaagcgg
     9721 gaccgcagtg aaagcaggag actttctaga aaaaaacacc agttgtcaac cttggggcag
     9781 gcaggaatcc tgaagacgga cggcactcct cctcctgctg cctcaccctc tggcagcccg
     9841 tgagaagtac cggaagcgag ggcggggccg cgggatggcg agggagcggc agggactgaa
     9901 ctctctccaa acccaccctg acagggaaat gggccccgcc tgtgtcttgg gaactcagag
     9961 gctgaggtca ggcatgatgg ctcacgcctg taattccagc actttgggag gcagaggcgg
    10021 gtggatcacg acgtcaggag ttcaaggcaa gcctggccaa catggtgaaa ccccatctct
    10081 actaaaaatg caaaaattag ccaggtgtgg tggcgggcgc cttggaggct gaggcaagat
    10141 aatcacttga acctgggagg cggaggttgt agtgagccaa aaaaaaaaaa aaaagaaata
    10201 gctgaagtca cagtaggaga gaagctgctg agcctccagc accctgactc tagggccttg
    10261 gctttatgtc tatctgcagt atttttgtga tttttaaaaa ttcactttct tgttgcggtg
    10321 taacttacac agggtcaaat gcacaaatca tggccctgac tttagataaa aatctgcccc
    10381 cacaaccctt ctgttccttg ccagttttta aactgcctct aaccagggga accaccagag
    10441 ctggtggcct tgggaggttt cagccctccc gtcatgaatg gacatagctc atccaactgc
    10501 caagggagag agctgtgggt ctgggccagc cccaccaggt aactcccaaa gggcagcccc
    10561 acagcaagat gtgacccagt cattgcctga gggtctctgg ggctgtgttc caacctttct
    10621 ccccgctgtg tccccctgga aggccccatg cccaggggag gcgcctaccg gtctccagac
    10681 tgggtgatga gccggcggca ggtctccatc tgcacgtcca ggccgcgctt catgctgcac
    10741 atctccatgt actcgtgcag gtgccggttc atgtcgttct tggccgtggc caactccagc
    10801 tgtggtgggg caggcagggc catcggggtt aacaggtggc gttcacagcg cctctgttgc
    10861 ccccgccagg aggccaacac gccaagagca gtggctgggc cgggggccca ggcagccatt
    10921 actgaaggct cagattttaa aataaaccag accaaggcag aaggcagcga atacaccatt
    10981 gtttcctctg cttttcctgg aaatctttgc ctatggagga atgcaaaggt actgctgcag
    11041 gctgtgttct tttaagactg ataagagtgg tgaggaaagt cagtgtgggc gggatggagc
    11101 agatccacag gctcccaggg ccacacaggt tcctatgggg cccggctcac ccctcatccc
    11161 ctgtactgcg ctggtcctgg gctgtcccct cactgcactg tgagctcccg taagggttag
    11221 cgacaggttt tcctcatcct ggcagaccca gacctcgaag ggaattaact ctctctgtga
    11281 cccgtggcat acaagcctgg ctaactgctg aaaggggccc aatcctctta ccattctctc
    11341 cttatcactt tattttctca cctaaaagcc attttcagac actaaaagga agactcccat
    11401 attggaggtg ccattgattc tgagacctgt cctgatttca gaaatgtcac acgtggaaaa
    11461 cacggggctc agaatcaaag aaatccggtc tccctccccg ggcccattct gcagcccaca
    11521 ctctgaggca ggcggagagg cagggcccag gcagcacaaa ctcagtgcca gggacagtcg
    11581 attaattaag cgatcgcagg ccgcccgcgg tggagctcca attcgcccta tagtgagtcg
    11641 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    11701 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    11761 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa
    11821 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
    11881 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
    11941 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    12001 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    12061 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    12121 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    12181 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    12241 cgcttaatgc gccgctacag ggcgcgtcag
//
