LOCUS       sequence-156571-       11308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11308)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 11308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-GFP
FEATURES             Location/Qualifiers
     source          1..11308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5641..5643,5644..5646,5647..6360)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5641..5643,5644..5646,5647..6357)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5686..5707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5947..5966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6294..6315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10664..10683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10665..10683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10693..10710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10693..10709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10702..10724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10850..11305)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10987..11008)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11199..11218
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgcgt
     5641 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5701 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5761 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5821 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5881 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5941 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6001 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6061 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6121 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6181 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6241 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6301 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6361 ggatccactg agttatcgat aagcttagat cgagcacaag aggaagagag agaccctcac
     6421 tgctggggag tccctgccac actcagtccc ccaccacact gaatctcccc tcctcacagt
     6481 tgccatgtag accccttgaa gaggggaggg gcctagggag ccgcaccttg tcatgtacca
     6541 tcaataaagt accctgtgct caaccagtta cttgtcctgt cttattctag ggtctggggc
     6601 agaggggagg gaagctgggc ttgtgtcaag gtgagacatt cttgctgggg agggacctgg
     6661 tatgttctcc tcagactgag ggtagggcct ccaaacagcc ttgcttgctt cgagaaccat
     6721 ttgcttcccg ctcagacgtc ttgagtgcta caggaagctg gcaccactac ttcagagaac
     6781 aaggcctttt cctctcctcg ctccagtcct aggctatctg ctgttggcca aacatggaag
     6841 aagctattct gtgggcagcc ccagggaggc tgacaggtgg aggaagtcag ggctcgcact
     6901 gggctctgac gctgactggt tagtggagct cagcctggag ctgagctgca gcgggcaatt
     6961 ccagcttggc ctccgcagct gtgaggtctt gagcacgtgc tctattgctt tctgtgccct
     7021 cgtgtcttat ctgaggacat cgtggccagc ccctaaggtc ttcaagcagg attcatctag
     7081 gtaaaccaag tacctaaaac catgcccaag gcggtaagga ctatataatg tttaaaaatc
     7141 ggtaaaaatg cccacctcgc atagttttga ggaagatgaa ctgagatgtg tcagggtgac
     7201 ttatttccat catcgtcctt aggggaactt gggtaggggc aaggcgtgta gctgggacct
     7261 aggtccagac ccctggctct gccactgaac ggctcagttg ctttgggcag ttactcccgg
     7321 gcctcacttt gcacgtgtgc ttacctagtg gagacaaaag tacatacctc ggtagagcgc
     7381 gcacgcctgt aaccccagca ctttgggagg ccaaggtggg tgtatcacct gaggtcagga
     7441 gtttgagacc agcctggcca acatggtgaa actccgtctc tactaaaatt acaaaaatca
     7501 gccaggcttc atggcacatg cctatagtcc cagctacagg catgctgaag caggagaatc
     7561 gcttgcaccc cggaggcaga ggctgcagtg agctgagacc acaccactgc actccagcct
     7621 aggcaacaga gtatgagact ccatctcaaa aaaaaaaaaa gtacctacct cagagttcaa
     7681 actagtgaat attaggaagt gcttgagaca gtgacaccaa agtgcacaat aaatactcgc
     7741 cagtttcatt attattaaag aatccatttg aatgtcagct caacacagcc tcctataccg
     7801 aggcattgtg aaccgcatct ccccagcttc tccaggcttt tccaagaatc agggacactg
     7861 tagcctgttg gtctcagtgt atgacagaca cggaggaagc acatctttag ctgatactta
     7921 aacagagacc ctgagcgcac atacacccgc gcacacatgc atggagcttc accttctctg
     7981 tcattctgca gtgaccagga gagcaagagc tcccacctcc cttcaaaaca ctgtgcccat
     8041 cccgggcact aaggcctctt taaagcacgg cacctccacg agggagggcc acagccacat
     8101 acactccacc tggcaggtgg acagcgtgag cacgtggacc atagcaggga caaggtgccc
     8161 cggccagccc caacgccctc tgccgctgac agggacagaa gccctctcca gctgcgtgtg
     8221 ctgcagaggc catgcgtagc ctccagctgc attctattcc actccagtgc ctgggccagt
     8281 tagcaccagt gtggaagaca gtgagctggc tccggacaac agggatggag gaaaggtccc
     8341 acattcacat tcctgatacg tggacaaggt gaggggccgc aatcgctctg gcagcatttt
     8401 aaagatgggg aagtagcaga cacccacgcg tgaaggcagg agagccccaa ctgtggtgga
     8461 aatggcccca gaatggtagg gccaagccta gctccagaca ccccagagcc ctggagaagc
     8521 caagactgag ggagaaagcc tgagggagga gcgccccagt ccccagggac cggcctggtg
     8581 cagagctgca gctgatgttc ccctctgtgc agccccaccc tctgcctcgc tgagctccct
     8641 gctgcgaggg cctcgggtgc aagggggagg caggtctcta tctcatggag ctgtcagatg
     8701 agacatcgcg atcggagtcc tcagcctcgc ttggcggcgg cggcgggtcg ctaagcggga
     8761 ccgcagtgaa agcaggagac tttctagaaa aaaacaccag ttgtcaacct tggggcaggc
     8821 aggaatcctg aagacggacg gcactcctcc tcctgctgcc tcaccctctg gcagcccgtg
     8881 agaagtaccg gaagcgaggg cggggccgcg ggatggcgag ggagcggcag ggactgaact
     8941 ctctccaaac ccaccctgac agggaaatgg gccccgcctg tgtcttggga actcagaggc
     9001 tgaggtcagg catgatggct cacgcctgta attccagcac tttgggaggc agaggcgggt
     9061 ggatcacgac gtcaggagtt caaggcaagc ctggccaaca tggtgaaacc ccatctctac
     9121 taaaaatgca aaaattagcc aggtgtggtg gcgggcgcct tggaggctga ggcaagataa
     9181 tcacttgaac ctgggaggcg gaggttgtag tgagccaaaa aaaaaaaaaa aagaaatagc
     9241 tgaagtcaca gtaggagaga agctgctgag cctccagcac cctgactcta gggccttggc
     9301 tttatgtcta tctgcagtat ttttgtgatt tttaaaaatt cactttcttg ttgcggtgta
     9361 acttacacag ggtcaaatgc acaaatcatg gccctgactt tagataaaaa tctgccccca
     9421 caacccttct gttccttgcc agtttttaaa ctgcctctaa ccaggggaac caccagagct
     9481 ggtggccttg ggaggtttca gccctcccgt catgaatgga catagctcat ccaactgcca
     9541 agggagagag ctgtgggtct gggccagccc caccaggtaa ctcccaaagg gcagccccac
     9601 agcaagatgt gacccagtca ttgcctgagg gtctctgggg ctgtgttcca acctttctcc
     9661 ccgctgtgtc cccctggaag gccccatgcc caggggaggc gcctaccggt ctccagactg
     9721 ggtgatgagc cggcggcagg tctccatctg cacgtccagg ccgcgcttca tgctgcacat
     9781 ctccatgtac tcgtgcaggt gccggttcat gtcgttcttg gccgtggcca actccagctg
     9841 tggtggggca ggcagggcca tcggggttaa caggtggcgt tcacagcgcc tctgttgccc
     9901 ccgccaggag gccaacacgc caagagcagt ggctgggccg ggggcccagg cagccattac
     9961 tgaaggctca gattttaaaa taaaccagac caaggcagaa ggcagcgaat acaccattgt
    10021 ttcctctgct tttcctggaa atctttgcct atggaggaat gcaaaggtac tgctgcaggc
    10081 tgtgttcttt taagactgat aagagtggtg aggaaagtca gtgtgggcgg gatggagcag
    10141 atccacaggc tcccagggcc acacaggttc ctatggggcc cggctcaccc ctcatcccct
    10201 gtactgcgct ggtcctgggc tgtcccctca ctgcactgtg agctcccgta agggttagcg
    10261 acaggttttc ctcatcctgg cagacccaga cctcgaaggg aattaactct ctctgtgacc
    10321 cgtggcatac aagcctggct aactgctgaa aggggcccaa tcctcttacc attctctcct
    10381 tatcacttta ttttctcacc taaaagccat tttcagacac taaaaggaag actcccatat
    10441 tggaggtgcc attgattctg agacctgtcc tgatttcaga aatgtcacac gtggaaaaca
    10501 cggggctcag aatcaaagaa atccggtctc cctccccggg cccattctgc agcccacact
    10561 ctgaggcagg cggagaggca gggcccaggc agcacaaact cagtgccagg gacagtcgat
    10621 taattaagcg atcgcaggcc gcccgcggtg gagctccaat tcgccctata gtgagtcgta
    10681 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    10741 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    10801 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
    10861 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
    10921 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
    10981 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
    11041 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
    11101 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
    11161 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
    11221 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
    11281 cttaatgcgc cgctacaggg cgcgtcag
//
