LOCUS       sequence-156572-       12953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the mCherry gene to the GAPDH locus of human cells.
            mCherry is expressed from a 2A sequence at the C terminus of GAPDH. 
            Hygromycin B resistance is encoded by the Mygro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-mCherry-IRESMygro
FEATURES             Location/Qualifiers
     source          1..12953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5650..6357
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5795..5813)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6063..6082
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(6387..6961,6962..6964,6965..6973)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6554..6571)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6781..6800
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6983..8008
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12309..12328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12310..12328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12338..12355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12338..12354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12347..12369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12495..12950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12632..12653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12844..12863
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgcgc
     5641 caagtcgaca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     5701 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     5761 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     5821 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     5881 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     5941 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     6001 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     6061 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     6121 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     6181 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     6241 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     6301 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtct
     6361 agatcataat cagcggatcg gatccgcccc tctccctccc ccccccctaa cgttactggc
     6421 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6481 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6541 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6601 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6661 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6721 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6781 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6841 atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa
     6901 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6961 tatggccaca accatggcgc gtatgaaaaa gcccgagctg actgccacct ctgtcgagaa
     7021 gttcctgatt gaaaagtttg attccgtgag tgacctgatg cagctgagcg agggcgagga
     7081 atcccgggcc ttctcttttg acgtgggcgg aaggggctac gtgctgagag tcaactcttg
     7141 cgccgacgga ttctataagg atcgctacgt gtatcgacac tttgctagtg ccgctctgcc
     7201 catccctgag gtgctggaca ttggcgagtt cagcgaatcc ctgacatact gtatctccag
     7261 gagagcccag ggagtgactc tgcaggatct gcctgagacc gaactgcctg ccgtgctgca
     7321 gcctgtggct gaagctatgg acgctatcgc agccgctgat ctgtcacaga ctagcgggtt
     7381 cggcccattt ggcccccagg gaattgggca gtacaccaca tggagagact ttatctgcgc
     7441 aattgccgat ccccacgtgt atcattggca gacagtcatg gacgataccg tgagcgccag
     7501 tgtcgctcag gcactggacg agctgatgct gtgggcagag gattgtcctg aagtgaggca
     7561 cctggtccat gctgacttcg ggagcaacaa tgtgctgacc gataacggca gaatcacagc
     7621 cgtcattgac tggagtgagg ctatgtttgg cgattcacag tacgaagtgg ctaatatctt
     7681 cttttggcga ccctggctgg catgcatgga gcagcagacc cgctatttcg agcggcgcca
     7741 tcctgaactg gcagggtctc caagactgcg ggcctacatg ctgcggattg gcctggacca
     7801 gctgtatcag agcctggtgg atggaaattt tgacgatgca gcatgggcac aggggcggtg
     7861 cgacgctatc gtgcgcagcg gagcaggaac tgtgggaaga acccagattg cccgaaggtc
     7921 cgctgcagtg tggacagatg ggtgcgtgga ggtgctggca gatagcggaa ataggagacc
     7981 aagtacaaga ccacgggcaa aagagtaatc tagacgcgta tcgataagct tagatcgagc
     8041 acaagaggaa gagagagacc ctcactgctg gggagtccct gccacactca gtcccccacc
     8101 acactgaatc tcccctcctc acagttgcca tgtagacccc ttgaagaggg gaggggccta
     8161 gggagccgca ccttgtcatg taccatcaat aaagtaccct gtgctcaacc agttacttgt
     8221 cctgtcttat tctagggtct ggggcagagg ggagggaagc tgggcttgtg tcaaggtgag
     8281 acattcttgc tggggaggga cctggtatgt tctcctcaga ctgagggtag ggcctccaaa
     8341 cagccttgct tgcttcgaga accatttgct tcccgctcag acgtcttgag tgctacagga
     8401 agctggcacc actacttcag agaacaaggc cttttcctct cctcgctcca gtcctaggct
     8461 atctgctgtt ggccaaacat ggaagaagct attctgtggg cagccccagg gaggctgaca
     8521 ggtggaggaa gtcagggctc gcactgggct ctgacgctga ctggttagtg gagctcagcc
     8581 tggagctgag ctgcagcggg caattccagc ttggcctccg cagctgtgag gtcttgagca
     8641 cgtgctctat tgctttctgt gccctcgtgt cttatctgag gacatcgtgg ccagccccta
     8701 aggtcttcaa gcaggattca tctaggtaaa ccaagtacct aaaaccatgc ccaaggcggt
     8761 aaggactata taatgtttaa aaatcggtaa aaatgcccac ctcgcatagt tttgaggaag
     8821 atgaactgag atgtgtcagg gtgacttatt tccatcatcg tccttagggg aacttgggta
     8881 ggggcaaggc gtgtagctgg gacctaggtc cagacccctg gctctgccac tgaacggctc
     8941 agttgctttg ggcagttact cccgggcctc actttgcacg tgtgcttacc tagtggagac
     9001 aaaagtacat acctcggtag agcgcgcacg cctgtaaccc cagcactttg ggaggccaag
     9061 gtgggtgtat cacctgaggt caggagtttg agaccagcct ggccaacatg gtgaaactcc
     9121 gtctctacta aaattacaaa aatcagccag gcttcatggc acatgcctat agtcccagct
     9181 acaggcatgc tgaagcagga gaatcgcttg caccccggag gcagaggctg cagtgagctg
     9241 agaccacacc actgcactcc agcctaggca acagagtatg agactccatc tcaaaaaaaa
     9301 aaaaagtacc tacctcagag ttcaaactag tgaatattag gaagtgcttg agacagtgac
     9361 accaaagtgc acaataaata ctcgccagtt tcattattat taaagaatcc atttgaatgt
     9421 cagctcaaca cagcctccta taccgaggca ttgtgaaccg catctcccca gcttctccag
     9481 gcttttccaa gaatcaggga cactgtagcc tgttggtctc agtgtatgac agacacggag
     9541 gaagcacatc tttagctgat acttaaacag agaccctgag cgcacataca cccgcgcaca
     9601 catgcatgga gcttcacctt ctctgtcatt ctgcagtgac caggagagca agagctccca
     9661 cctcccttca aaacactgtg cccatcccgg gcactaaggc ctctttaaag cacggcacct
     9721 ccacgaggga gggccacagc cacatacact ccacctggca ggtggacagc gtgagcacgt
     9781 ggaccatagc agggacaagg tgccccggcc agccccaacg ccctctgccg ctgacaggga
     9841 cagaagccct ctccagctgc gtgtgctgca gaggccatgc gtagcctcca gctgcattct
     9901 attccactcc agtgcctggg ccagttagca ccagtgtgga agacagtgag ctggctccgg
     9961 acaacaggga tggaggaaag gtcccacatt cacattcctg atacgtggac aaggtgaggg
    10021 gccgcaatcg ctctggcagc attttaaaga tggggaagta gcagacaccc acgcgtgaag
    10081 gcaggagagc cccaactgtg gtggaaatgg ccccagaatg gtagggccaa gcctagctcc
    10141 agacacccca gagccctgga gaagccaaga ctgagggaga aagcctgagg gaggagcgcc
    10201 ccagtcccca gggaccggcc tggtgcagag ctgcagctga tgttcccctc tgtgcagccc
    10261 caccctctgc ctcgctgagc tccctgctgc gagggcctcg ggtgcaaggg ggaggcaggt
    10321 ctctatctca tggagctgtc agatgagaca tcgcgatcgg agtcctcagc ctcgcttggc
    10381 ggcggcggcg ggtcgctaag cgggaccgca gtgaaagcag gagactttct agaaaaaaac
    10441 accagttgtc aaccttgggg caggcaggaa tcctgaagac ggacggcact cctcctcctg
    10501 ctgcctcacc ctctggcagc ccgtgagaag taccggaagc gagggcgggg ccgcgggatg
    10561 gcgagggagc ggcagggact gaactctctc caaacccacc ctgacaggga aatgggcccc
    10621 gcctgtgtct tgggaactca gaggctgagg tcaggcatga tggctcacgc ctgtaattcc
    10681 agcactttgg gaggcagagg cgggtggatc acgacgtcag gagttcaagg caagcctggc
    10741 caacatggtg aaaccccatc tctactaaaa atgcaaaaat tagccaggtg tggtggcggg
    10801 cgccttggag gctgaggcaa gataatcact tgaacctggg aggcggaggt tgtagtgagc
    10861 caaaaaaaaa aaaaaaagaa atagctgaag tcacagtagg agagaagctg ctgagcctcc
    10921 agcaccctga ctctagggcc ttggctttat gtctatctgc agtatttttg tgatttttaa
    10981 aaattcactt tcttgttgcg gtgtaactta cacagggtca aatgcacaaa tcatggccct
    11041 gactttagat aaaaatctgc ccccacaacc cttctgttcc ttgccagttt ttaaactgcc
    11101 tctaaccagg ggaaccacca gagctggtgg ccttgggagg tttcagccct cccgtcatga
    11161 atggacatag ctcatccaac tgccaaggga gagagctgtg ggtctgggcc agccccacca
    11221 ggtaactccc aaagggcagc cccacagcaa gatgtgaccc agtcattgcc tgagggtctc
    11281 tggggctgtg ttccaacctt tctccccgct gtgtccccct ggaaggcccc atgcccaggg
    11341 gaggcgccta ccggtctcca gactgggtga tgagccggcg gcaggtctcc atctgcacgt
    11401 ccaggccgcg cttcatgctg cacatctcca tgtactcgtg caggtgccgg ttcatgtcgt
    11461 tcttggccgt ggccaactcc agctgtggtg gggcaggcag ggccatcggg gttaacaggt
    11521 ggcgttcaca gcgcctctgt tgcccccgcc aggaggccaa cacgccaaga gcagtggctg
    11581 ggccgggggc ccaggcagcc attactgaag gctcagattt taaaataaac cagaccaagg
    11641 cagaaggcag cgaatacacc attgtttcct ctgcttttcc tggaaatctt tgcctatgga
    11701 ggaatgcaaa ggtactgctg caggctgtgt tcttttaaga ctgataagag tggtgaggaa
    11761 agtcagtgtg ggcgggatgg agcagatcca caggctccca gggccacaca ggttcctatg
    11821 gggcccggct cacccctcat cccctgtact gcgctggtcc tgggctgtcc cctcactgca
    11881 ctgtgagctc ccgtaagggt tagcgacagg ttttcctcat cctggcagac ccagacctcg
    11941 aagggaatta actctctctg tgacccgtgg catacaagcc tggctaactg ctgaaagggg
    12001 cccaatcctc ttaccattct ctccttatca ctttattttc tcacctaaaa gccattttca
    12061 gacactaaaa ggaagactcc catattggag gtgccattga ttctgagacc tgtcctgatt
    12121 tcagaaatgt cacacgtgga aaacacgggg ctcagaatca aagaaatccg gtctccctcc
    12181 ccgggcccat tctgcagccc acactctgag gcaggcggag aggcagggcc caggcagcac
    12241 aaactcagtg ccagggacag tcgattaatt aagcgatcgc aggccgcccg cggtggagct
    12301 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    12361 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    12421 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    12481 tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
    12541 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    12601 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    12661 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
    12721 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
    12781 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
    12841 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    12901 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cag
//
