LOCUS       sequence-156573-       12521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the eGFP gene to the GAPDH locus of human cells. 
            eGFP is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-eGFP-IRESMuro
FEATURES             Location/Qualifiers
     source          1..12521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5641..5643,5644..5646,5647..6360)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5641..5643,5644..5646,5647..6357)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5686..5707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5947..5966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6294..6315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6368..6942,6943..6945,6946..6954)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6535..6552)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6762..6781
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(11877..11896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11878..11896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11906..11923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11906..11922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11915..11937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12063..12518)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12200..12221)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12412..12431
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgcgt
     5641 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     5701 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     5761 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     5821 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     5881 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5941 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6001 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6061 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     6121 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     6181 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     6241 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6301 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     6361 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     6421 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6481 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6541 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6601 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6661 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6721 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6781 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6841 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6901 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggcg
     6961 cgtatggcaa gactgaccga atacaagcct accgtgagac tggcaacaag agatgatgtc
     7021 ccaagagccg tgcgaaccct ggcagcagca ttcgcagact acccagccac aagacacact
     7081 gtggaccccg ataggcatat cgagagagtg accgagctgc aggaactgtt tctgacacgg
     7141 gtgggcctgg atattggaaa ggtctgggtg gcagatgatg gggctgctgt ggccgtctgg
     7201 acaaccccag agtctgtgga agctggagct gtcttcgctg agatcggccc tcgaatggca
     7261 gaactgtctg gctccaggct ggcagcacag cagcagatgg agggactgct ggcaccacac
     7321 agacctaagg aaccagcttg gttcctggca actgtggggg tctctcccga ccatcagggc
     7381 aaaggactgg ggagtgccgt ggtcctgcct ggagtggagg ctgcagaaag agctggagtc
     7441 ccagcttttc tggagacatc tgccccccgg aacctgcctt tctatgaacg cctgggcttt
     7501 actgtgaccg ctgacgtgga ggtgcccgaa ggcccccgaa cttggtgcat gacccgaaag
     7561 ccaggagcat aaatctagag gcgcgccatc gataagctta gatcgagcac aagaggaaga
     7621 gagagaccct cactgctggg gagtccctgc cacactcagt cccccaccac actgaatctc
     7681 ccctcctcac agttgccatg tagacccctt gaagagggga ggggcctagg gagccgcacc
     7741 ttgtcatgta ccatcaataa agtaccctgt gctcaaccag ttacttgtcc tgtcttattc
     7801 tagggtctgg ggcagagggg agggaagctg ggcttgtgtc aaggtgagac attcttgctg
     7861 gggagggacc tggtatgttc tcctcagact gagggtaggg cctccaaaca gccttgcttg
     7921 cttcgagaac catttgcttc ccgctcagac gtcttgagtg ctacaggaag ctggcaccac
     7981 tacttcagag aacaaggcct tttcctctcc tcgctccagt cctaggctat ctgctgttgg
     8041 ccaaacatgg aagaagctat tctgtgggca gccccaggga ggctgacagg tggaggaagt
     8101 cagggctcgc actgggctct gacgctgact ggttagtgga gctcagcctg gagctgagct
     8161 gcagcgggca attccagctt ggcctccgca gctgtgaggt cttgagcacg tgctctattg
     8221 ctttctgtgc cctcgtgtct tatctgagga catcgtggcc agcccctaag gtcttcaagc
     8281 aggattcatc taggtaaacc aagtacctaa aaccatgccc aaggcggtaa ggactatata
     8341 atgtttaaaa atcggtaaaa atgcccacct cgcatagttt tgaggaagat gaactgagat
     8401 gtgtcagggt gacttatttc catcatcgtc cttaggggaa cttgggtagg ggcaaggcgt
     8461 gtagctggga cctaggtcca gacccctggc tctgccactg aacggctcag ttgctttggg
     8521 cagttactcc cgggcctcac tttgcacgtg tgcttaccta gtggagacaa aagtacatac
     8581 ctcggtagag cgcgcacgcc tgtaacccca gcactttggg aggccaaggt gggtgtatca
     8641 cctgaggtca ggagtttgag accagcctgg ccaacatggt gaaactccgt ctctactaaa
     8701 attacaaaaa tcagccaggc ttcatggcac atgcctatag tcccagctac aggcatgctg
     8761 aagcaggaga atcgcttgca ccccggaggc agaggctgca gtgagctgag accacaccac
     8821 tgcactccag cctaggcaac agagtatgag actccatctc aaaaaaaaaa aaagtaccta
     8881 cctcagagtt caaactagtg aatattagga agtgcttgag acagtgacac caaagtgcac
     8941 aataaatact cgccagtttc attattatta aagaatccat ttgaatgtca gctcaacaca
     9001 gcctcctata ccgaggcatt gtgaaccgca tctccccagc ttctccaggc ttttccaaga
     9061 atcagggaca ctgtagcctg ttggtctcag tgtatgacag acacggagga agcacatctt
     9121 tagctgatac ttaaacagag accctgagcg cacatacacc cgcgcacaca tgcatggagc
     9181 ttcaccttct ctgtcattct gcagtgacca ggagagcaag agctcccacc tcccttcaaa
     9241 acactgtgcc catcccgggc actaaggcct ctttaaagca cggcacctcc acgagggagg
     9301 gccacagcca catacactcc acctggcagg tggacagcgt gagcacgtgg accatagcag
     9361 ggacaaggtg ccccggccag ccccaacgcc ctctgccgct gacagggaca gaagccctct
     9421 ccagctgcgt gtgctgcaga ggccatgcgt agcctccagc tgcattctat tccactccag
     9481 tgcctgggcc agttagcacc agtgtggaag acagtgagct ggctccggac aacagggatg
     9541 gaggaaaggt cccacattca cattcctgat acgtggacaa ggtgaggggc cgcaatcgct
     9601 ctggcagcat tttaaagatg gggaagtagc agacacccac gcgtgaaggc aggagagccc
     9661 caactgtggt ggaaatggcc ccagaatggt agggccaagc ctagctccag acaccccaga
     9721 gccctggaga agccaagact gagggagaaa gcctgaggga ggagcgcccc agtccccagg
     9781 gaccggcctg gtgcagagct gcagctgatg ttcccctctg tgcagcccca ccctctgcct
     9841 cgctgagctc cctgctgcga gggcctcggg tgcaaggggg aggcaggtct ctatctcatg
     9901 gagctgtcag atgagacatc gcgatcggag tcctcagcct cgcttggcgg cggcggcggg
     9961 tcgctaagcg ggaccgcagt gaaagcagga gactttctag aaaaaaacac cagttgtcaa
    10021 ccttggggca ggcaggaatc ctgaagacgg acggcactcc tcctcctgct gcctcaccct
    10081 ctggcagccc gtgagaagta ccggaagcga gggcggggcc gcgggatggc gagggagcgg
    10141 cagggactga actctctcca aacccaccct gacagggaaa tgggccccgc ctgtgtcttg
    10201 ggaactcaga ggctgaggtc aggcatgatg gctcacgcct gtaattccag cactttggga
    10261 ggcagaggcg ggtggatcac gacgtcagga gttcaaggca agcctggcca acatggtgaa
    10321 accccatctc tactaaaaat gcaaaaatta gccaggtgtg gtggcgggcg ccttggaggc
    10381 tgaggcaaga taatcacttg aacctgggag gcggaggttg tagtgagcca aaaaaaaaaa
    10441 aaaaagaaat agctgaagtc acagtaggag agaagctgct gagcctccag caccctgact
    10501 ctagggcctt ggctttatgt ctatctgcag tatttttgtg atttttaaaa attcactttc
    10561 ttgttgcggt gtaacttaca cagggtcaaa tgcacaaatc atggccctga ctttagataa
    10621 aaatctgccc ccacaaccct tctgttcctt gccagttttt aaactgcctc taaccagggg
    10681 aaccaccaga gctggtggcc ttgggaggtt tcagccctcc cgtcatgaat ggacatagct
    10741 catccaactg ccaagggaga gagctgtggg tctgggccag ccccaccagg taactcccaa
    10801 agggcagccc cacagcaaga tgtgacccag tcattgcctg agggtctctg gggctgtgtt
    10861 ccaacctttc tccccgctgt gtccccctgg aaggccccat gcccagggga ggcgcctacc
    10921 ggtctccaga ctgggtgatg agccggcggc aggtctccat ctgcacgtcc aggccgcgct
    10981 tcatgctgca catctccatg tactcgtgca ggtgccggtt catgtcgttc ttggccgtgg
    11041 ccaactccag ctgtggtggg gcaggcaggg ccatcggggt taacaggtgg cgttcacagc
    11101 gcctctgttg cccccgccag gaggccaaca cgccaagagc agtggctggg ccgggggccc
    11161 aggcagccat tactgaaggc tcagatttta aaataaacca gaccaaggca gaaggcagcg
    11221 aatacaccat tgtttcctct gcttttcctg gaaatctttg cctatggagg aatgcaaagg
    11281 tactgctgca ggctgtgttc ttttaagact gataagagtg gtgaggaaag tcagtgtggg
    11341 cgggatggag cagatccaca ggctcccagg gccacacagg ttcctatggg gcccggctca
    11401 cccctcatcc cctgtactgc gctggtcctg ggctgtcccc tcactgcact gtgagctccc
    11461 gtaagggtta gcgacaggtt ttcctcatcc tggcagaccc agacctcgaa gggaattaac
    11521 tctctctgtg acccgtggca tacaagcctg gctaactgct gaaaggggcc caatcctctt
    11581 accattctct ccttatcact ttattttctc acctaaaagc cattttcaga cactaaaagg
    11641 aagactccca tattggaggt gccattgatt ctgagacctg tcctgatttc agaaatgtca
    11701 cacgtggaaa acacggggct cagaatcaaa gaaatccggt ctccctcccc gggcccattc
    11761 tgcagcccac actctgaggc aggcggagag gcagggccca ggcagcacaa actcagtgcc
    11821 agggacagtc gattaattaa gcgatcgcag gccgcccgcg gtggagctcc aattcgccct
    11881 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    11941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    12001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    12061 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    12121 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
    12181 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    12241 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    12301 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    12361 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    12421 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    12481 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca g
//
