LOCUS       sequence-156577-       14915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the LacZ gene to the GAPDH locus of human cells. 
            LacZ is expressed from a 2A sequence at the C terminus of GAPDH. 
            Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-LacZ-IRESMuro
FEATURES             Location/Qualifiers
     source          1..14915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5644..5664
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5695..8742
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     misc_feature    join(8762..9336,9337..9339,9340..9348)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8929..8946)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9156..9175
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(14271..14290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14272..14290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14300..14317)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14300..14316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(14457..14912)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14594..14615)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14806..14825
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgatg
     5641 gctccaaaaa agaagagaaa ggtagaagac ccccgcatgg ctcgcgatga tcccgtcgtt
     5701 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5761 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5821 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     5881 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     5941 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
     6001 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     6061 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
     6121 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
     6181 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt
     6241 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
     6301 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
     6361 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
     6421 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
     6481 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
     6541 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
     6601 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
     6661 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
     6721 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
     6781 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
     6841 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
     6901 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
     6961 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
     7021 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
     7081 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
     7141 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
     7201 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
     7261 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
     7321 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
     7381 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
     7441 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
     7501 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
     7561 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
     7621 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
     7681 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
     7741 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
     7801 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
     7861 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
     7921 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
     7981 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
     8041 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
     8101 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
     8161 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
     8221 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
     8281 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
     8341 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
     8401 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
     8461 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
     8521 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
     8581 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
     8641 atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
     8701 gccggtcgct accattacca gttggtctgg tgtcaaaaat aacgccaagt cgacggatcc
     8761 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     8821 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     8881 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     8941 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     9001 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     9061 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     9121 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     9181 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     9241 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     9301 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggcgcgtatg
     9361 gcaagactga ccgaatacaa gcctaccgtg agactggcaa caagagatga tgtcccaaga
     9421 gccgtgcgaa ccctggcagc agcattcgca gactacccag ccacaagaca cactgtggac
     9481 cccgataggc atatcgagag agtgaccgag ctgcaggaac tgtttctgac acgggtgggc
     9541 ctggatattg gaaaggtctg ggtggcagat gatggggctg ctgtggccgt ctggacaacc
     9601 ccagagtctg tggaagctgg agctgtcttc gctgagatcg gccctcgaat ggcagaactg
     9661 tctggctcca ggctggcagc acagcagcag atggagggac tgctggcacc acacagacct
     9721 aaggaaccag cttggttcct ggcaactgtg ggggtctctc ccgaccatca gggcaaagga
     9781 ctggggagtg ccgtggtcct gcctggagtg gaggctgcag aaagagctgg agtcccagct
     9841 tttctggaga catctgcccc ccggaacctg cctttctatg aacgcctggg ctttactgtg
     9901 accgctgacg tggaggtgcc cgaaggcccc cgaacttggt gcatgacccg aaagccagga
     9961 gcataaatct agaggcgcgc catcgataag cttagatcga gcacaagagg aagagagaga
    10021 ccctcactgc tggggagtcc ctgccacact cagtccccca ccacactgaa tctcccctcc
    10081 tcacagttgc catgtagacc ccttgaagag gggaggggcc tagggagccg caccttgtca
    10141 tgtaccatca ataaagtacc ctgtgctcaa ccagttactt gtcctgtctt attctagggt
    10201 ctggggcaga ggggagggaa gctgggcttg tgtcaaggtg agacattctt gctggggagg
    10261 gacctggtat gttctcctca gactgagggt agggcctcca aacagccttg cttgcttcga
    10321 gaaccatttg cttcccgctc agacgtcttg agtgctacag gaagctggca ccactacttc
    10381 agagaacaag gccttttcct ctcctcgctc cagtcctagg ctatctgctg ttggccaaac
    10441 atggaagaag ctattctgtg ggcagcccca gggaggctga caggtggagg aagtcagggc
    10501 tcgcactggg ctctgacgct gactggttag tggagctcag cctggagctg agctgcagcg
    10561 ggcaattcca gcttggcctc cgcagctgtg aggtcttgag cacgtgctct attgctttct
    10621 gtgccctcgt gtcttatctg aggacatcgt ggccagcccc taaggtcttc aagcaggatt
    10681 catctaggta aaccaagtac ctaaaaccat gcccaaggcg gtaaggacta tataatgttt
    10741 aaaaatcggt aaaaatgccc acctcgcata gttttgagga agatgaactg agatgtgtca
    10801 gggtgactta tttccatcat cgtccttagg ggaacttggg taggggcaag gcgtgtagct
    10861 gggacctagg tccagacccc tggctctgcc actgaacggc tcagttgctt tgggcagtta
    10921 ctcccgggcc tcactttgca cgtgtgctta cctagtggag acaaaagtac atacctcggt
    10981 agagcgcgca cgcctgtaac cccagcactt tgggaggcca aggtgggtgt atcacctgag
    11041 gtcaggagtt tgagaccagc ctggccaaca tggtgaaact ccgtctctac taaaattaca
    11101 aaaatcagcc aggcttcatg gcacatgcct atagtcccag ctacaggcat gctgaagcag
    11161 gagaatcgct tgcaccccgg aggcagaggc tgcagtgagc tgagaccaca ccactgcact
    11221 ccagcctagg caacagagta tgagactcca tctcaaaaaa aaaaaaagta cctacctcag
    11281 agttcaaact agtgaatatt aggaagtgct tgagacagtg acaccaaagt gcacaataaa
    11341 tactcgccag tttcattatt attaaagaat ccatttgaat gtcagctcaa cacagcctcc
    11401 tataccgagg cattgtgaac cgcatctccc cagcttctcc aggcttttcc aagaatcagg
    11461 gacactgtag cctgttggtc tcagtgtatg acagacacgg aggaagcaca tctttagctg
    11521 atacttaaac agagaccctg agcgcacata cacccgcgca cacatgcatg gagcttcacc
    11581 ttctctgtca ttctgcagtg accaggagag caagagctcc cacctccctt caaaacactg
    11641 tgcccatccc gggcactaag gcctctttaa agcacggcac ctccacgagg gagggccaca
    11701 gccacataca ctccacctgg caggtggaca gcgtgagcac gtggaccata gcagggacaa
    11761 ggtgccccgg ccagccccaa cgccctctgc cgctgacagg gacagaagcc ctctccagct
    11821 gcgtgtgctg cagaggccat gcgtagcctc cagctgcatt ctattccact ccagtgcctg
    11881 ggccagttag caccagtgtg gaagacagtg agctggctcc ggacaacagg gatggaggaa
    11941 aggtcccaca ttcacattcc tgatacgtgg acaaggtgag gggccgcaat cgctctggca
    12001 gcattttaaa gatggggaag tagcagacac ccacgcgtga aggcaggaga gccccaactg
    12061 tggtggaaat ggccccagaa tggtagggcc aagcctagct ccagacaccc cagagccctg
    12121 gagaagccaa gactgaggga gaaagcctga gggaggagcg ccccagtccc cagggaccgg
    12181 cctggtgcag agctgcagct gatgttcccc tctgtgcagc cccaccctct gcctcgctga
    12241 gctccctgct gcgagggcct cgggtgcaag ggggaggcag gtctctatct catggagctg
    12301 tcagatgaga catcgcgatc ggagtcctca gcctcgcttg gcggcggcgg cgggtcgcta
    12361 agcgggaccg cagtgaaagc aggagacttt ctagaaaaaa acaccagttg tcaaccttgg
    12421 ggcaggcagg aatcctgaag acggacggca ctcctcctcc tgctgcctca ccctctggca
    12481 gcccgtgaga agtaccggaa gcgagggcgg ggccgcggga tggcgaggga gcggcaggga
    12541 ctgaactctc tccaaaccca ccctgacagg gaaatgggcc ccgcctgtgt cttgggaact
    12601 cagaggctga ggtcaggcat gatggctcac gcctgtaatt ccagcacttt gggaggcaga
    12661 ggcgggtgga tcacgacgtc aggagttcaa ggcaagcctg gccaacatgg tgaaacccca
    12721 tctctactaa aaatgcaaaa attagccagg tgtggtggcg ggcgccttgg aggctgaggc
    12781 aagataatca cttgaacctg ggaggcggag gttgtagtga gccaaaaaaa aaaaaaaaag
    12841 aaatagctga agtcacagta ggagagaagc tgctgagcct ccagcaccct gactctaggg
    12901 ccttggcttt atgtctatct gcagtatttt tgtgattttt aaaaattcac tttcttgttg
    12961 cggtgtaact tacacagggt caaatgcaca aatcatggcc ctgactttag ataaaaatct
    13021 gcccccacaa cccttctgtt ccttgccagt ttttaaactg cctctaacca ggggaaccac
    13081 cagagctggt ggccttggga ggtttcagcc ctcccgtcat gaatggacat agctcatcca
    13141 actgccaagg gagagagctg tgggtctggg ccagccccac caggtaactc ccaaagggca
    13201 gccccacagc aagatgtgac ccagtcattg cctgagggtc tctggggctg tgttccaacc
    13261 tttctccccg ctgtgtcccc ctggaaggcc ccatgcccag gggaggcgcc taccggtctc
    13321 cagactgggt gatgagccgg cggcaggtct ccatctgcac gtccaggccg cgcttcatgc
    13381 tgcacatctc catgtactcg tgcaggtgcc ggttcatgtc gttcttggcc gtggccaact
    13441 ccagctgtgg tggggcaggc agggccatcg gggttaacag gtggcgttca cagcgcctct
    13501 gttgcccccg ccaggaggcc aacacgccaa gagcagtggc tgggccgggg gcccaggcag
    13561 ccattactga aggctcagat tttaaaataa accagaccaa ggcagaaggc agcgaataca
    13621 ccattgtttc ctctgctttt cctggaaatc tttgcctatg gaggaatgca aaggtactgc
    13681 tgcaggctgt gttcttttaa gactgataag agtggtgagg aaagtcagtg tgggcgggat
    13741 ggagcagatc cacaggctcc cagggccaca caggttccta tggggcccgg ctcacccctc
    13801 atcccctgta ctgcgctggt cctgggctgt cccctcactg cactgtgagc tcccgtaagg
    13861 gttagcgaca ggttttcctc atcctggcag acccagacct cgaagggaat taactctctc
    13921 tgtgacccgt ggcatacaag cctggctaac tgctgaaagg ggcccaatcc tcttaccatt
    13981 ctctccttat cactttattt tctcacctaa aagccatttt cagacactaa aaggaagact
    14041 cccatattgg aggtgccatt gattctgaga cctgtcctga tttcagaaat gtcacacgtg
    14101 gaaaacacgg ggctcagaat caaagaaatc cggtctccct ccccgggccc attctgcagc
    14161 ccacactctg aggcaggcgg agaggcaggg cccaggcagc acaaactcag tgccagggac
    14221 agtcgattaa ttaagcgatc gcaggccgcc cgcggtggag ctccaattcg ccctatagtg
    14281 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    14341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    14401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat
    14461 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    14521 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    14581 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    14641 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    14701 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    14761 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    14821 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    14881 cgccgcgctt aatgcgccgc tacagggcgc gtcag
//
