LOCUS       sequence-156578-       13657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH 
            locus of human cells. It is expressed from a 2A sequence at the C 
            terminus of GAPDH. Puromycin resistance is encoded by the Meo gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 13657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-Luc2-IRESMeo
FEATURES             Location/Qualifiers
     source          1..13657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5584..5637
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5644..7296
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    join(7304..7878,7879..7881,7882..7890)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7471..7488)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7698..7717
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(13013..13032)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13014..13032)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13042..13059)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13042..13058)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13051..13073)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(13199..13654)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13336..13357)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13548..13567
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatgnn
     2221 ngggaaggtg aaggtcggag tcaacgggtg agttcgcggg tggctggggg gccctgggct
     2281 gcgaccgccc ccgaaccgcg tctacgagcc ttgcgggctc cgggtctttg cagtcgtatg
     2341 ggggcagggt agctgttccc cgcaaggaga gctcaaggtc agcgctcgga cctggcggag
     2401 ccccgcaccc aggctgtggc gccctgtgca gctccgccct tgcggcgcca tctgcccgga
     2461 gcctccttcc cctagtcccc agaaacagga ggtccctact cccgcccgag atcccgaccc
     2521 ggacccctag gtgggggacg ctttctttcc tttcgcgctc tgcggggtca cgtgtcgcag
     2581 aggagcccct cccccacggc ctccggcacc gcaggccccg ggatgctagt gcgcagcggg
     2641 tgcatccctg tccggatgct gcgcctgcgg tagagcggcc gccatgttgc aaccgggaag
     2701 gaaatgaatg ggcagccgtt aggaaagcct gccggtgact aaccctgcgc tcctgcctcg
     2761 atgggtggag tcgcgtgtgg cggggaagtc aggtggagcg aggctagctg gcccgatttc
     2821 tcctccgggt gatgcttttc ctagattatt ctctggtaaa tcaaagaagt gggtttatgg
     2881 aggtcctctt gtgtcccctc cccgcagagg tgtggtggct gtggcatggt gccaagccgg
     2941 gagaagctga gtcatgggta gttggaaaag gacatttcca ccgcaaaatg gcccctctgg
     3001 tggtggcccc ttcctgcagc gccggctcac ctcacggccc cgcccttccc ctgccagcct
     3061 agcgttgacc cgaccccaaa ggccaggctg taaatgtcac cgggaggatt gggtgtctgg
     3121 gcgcctcggg gaacctgccc ttctccccat tccgtcttcc ggaaaccaga tctcccaccg
     3181 caccctggtc tgaggttaaa tatagctgct gacctttctg tagctggggg cctgggctgg
     3241 ggctctctcc catcccttct ccccacacac atgcacttac ctgtgctccc actcctgatt
     3301 tctggaaaag agctaggaag gacaggcaac ttggcaaatc aaagccctgg gactaggggg
     3361 ttaaaataca gcttcccctc ttcccacccg ccccagtctc tgtccctttt gtaggaggga
     3421 cttagagaag gggtgggctt gccctgtcca gttaatttct gacctttact cctgcccttt
     3481 gagtttgatg atgctgagtg tacaagcgtt ttctccctaa agggtgcagc tgagctaggc
     3541 agcagcaagc attcctgggg tggcatagtg gggtggtgaa taccatgtac aaagcttgtg
     3601 cccagactgt gggtggcagt gccccacatg gccgcttctc ctggaagggc ttcgtatgac
     3661 tgggggtgtt gggcagccct ggagccttca gttgcagcca tgccttaagc caggccagcc
     3721 tggcagggaa gctcaaggga gataaaattc aacctcttgg gccctcctgg gggtaaggag
     3781 atgctgcatt cgccctctta atggggaggt ggcctagggc tgctcacata ttctggagga
     3841 gcctcccctc ctcatgcctt cttgcctctt gtctcttaga tttggtcgta ttgggcgcct
     3901 ggtcaccagg gctgctttta actctggtaa agtggatatt gttgccatca atgacccctt
     3961 cattgacctc aactacatgg tgagtgctac atggtgagcc ccaaagctgg tgtgggagga
     4021 gccacctggc tgatgggcag ccccttcata ccctcacgta ttcccccagg tttacatgtt
     4081 ccaatatgat tccacccatg gcaaattcca tggcaccgtc aaggctgaga acgggaagct
     4141 tgtcatcaat ggaaatccca tcaccatctt ccaggagtga gtggaagaca gaatggaaga
     4201 aatgtgcttt ggggaggcaa ctaggatggt gtggctccct tgggtatatg gtaaccttgt
     4261 gtccctcaat atggtcctgt ccccatctcc cccccacccc cataggcgag atccctccaa
     4321 aatcaagtgg ggcgatgctg gcgctgagta cgtcgtggag tccactggcg tcttcaccac
     4381 catggagaag gctggggtga gtgcaggagg gcccgcggga ggggaagctg actcagccct
     4441 gcaaaggcag gacccgggtt cataactgtc tgcttctctg ctgtaggctc atttgcaggg
     4501 gggagccaaa agggtcatca tctctgcccc ctctgctgat gcccccatgt tcgtcatggg
     4561 tgtgaaccat gagaagtatg acaacagcct caagatcatc aggtgaggaa ggcagggccc
     4621 gtggagaagc ggccagcctg gcaccctatg gacacgctcc cctgacttgc gccccgctcc
     4681 ctctttcttt gcagcaatgc ctcctgcacc accaactgct tagcacccct ggccaaggtc
     4741 atccatgaca actttggtat cgtggaagga ctcatggtat gagagctggg gaatgggact
     4801 gaggctccca cctttctcat ccaagactgg ctcctccctg ccggggctgc gtgcaaccct
     4861 ggggttgggg gttctgggga ctggctttcc cataatttcc tttcaaggtg gggagggagg
     4921 tagaggggtg atgtggggag tacgctgcag ggcctcactc cttttgcaga ccacagtcca
     4981 tgccatcact gccacccaga agactgtgga tggcccctcc gggaaactgt ggcgtgatgg
     5041 ccgcggggct ctccagaaca tcatccctgc ctctactggc gctgccaagg ctgtgggcaa
     5101 ggtcatccct gagctgaacg ggaagctcac tggcatggcc ttccgtgtcc ccactgccaa
     5161 cgtgtcagtg gtggacctga cctgccgtct agaaaaacct gccaaatatg atgacatcaa
     5221 gaaggtggtg aagcaggcgt cggagggccc cctcaagggc atcctgggct acactgagca
     5281 ccaggtggtc tcctctgact tcaacagcga cacccactcc tccacctttg acgctggggc
     5341 tggcattgcc ctcaacgacc actttgtcaa gctcatttcc tggtatgtgg ctggggccag
     5401 agactggctc ttaaaaagtg cagggtctgg cgccctctgg tggctggctc agaaaaaggg
     5461 ccctgacaac tcttttcatc ttctaggtat gacaacgaat ttggctacag caacagggtg
     5521 gtggacctca tggcccacat ggcctccaag gagggaatgg cgcgtggcgg cgggtccgga
     5581 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc tggcccggcg
     5641 cgtatggaag atgccaaaaa cattaagaag ggcccagcgc cattctaccc actcgaagac
     5701 gggaccgccg gcgagcagct gcacaaagcc atgaagcgct acgccctggt gcccggcacc
     5761 atcgccttta ccgacgcaca tatcgaggtg gacattacct acgccgagta cttcgagatg
     5821 agcgttcggc tggcagaagc tatgaagcgc tatgggctga atacaaacca tcggatcgtg
     5881 gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt tgggtgccct gttcatcggt
     5941 gtggctgtgg ccccagctaa cgacatctac aacgagcgcg agctgctgaa cagcatgggc
     6001 atcagccagc ccaccgtcgt attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg
     6061 caaaagaagc taccgatcat acaaaagatc atcatcatgg atagcaagac cgactaccag
     6121 ggcttccaaa gcatgtacac cttcgtgact tcccatttgc cacccggctt caacgagtac
     6181 gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg ccctgatcat gaacagtagt
     6241 ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc
     6301 agtcatgccc gcgaccccat cttcggcaac cagatcatcc ccgacaccgc tatcctcagc
     6361 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
     6421 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
     6481 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
     6541 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
     6601 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
     6661 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
     6721 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
     6781 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
     6841 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
     6901 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
     6961 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
     7021 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
     7081 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
     7141 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     7201 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     7261 attaaggcca agaagggcgg caagatcgcc gtgtaaagat ccgcccctct ccctcccccc
     7321 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     7381 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     7441 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7501 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7561 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7621 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7681 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7741 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     7801 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     7861 gaaaaacacg atgataatat ggccacaacc atggcgcgta tggctcgggg cattgaacag
     7921 gatgggctgc acgccgggtc acctgctgct tgggtcgaaa gactgtttgg gtatgattgg
     7981 gctcagcaga caatcggctg ctctgacgcc gccgtgttca ggctgagtgc tcagggacgc
     8041 cccgtgctgt ttgtcaagac tgacctgtca ggggcactga acgagctgca ggatgaggca
     8101 gcccgcctga gctggctggc aaccacagga gtgccttgtg ctgcagtcct ggacgtggtc
     8161 actgaggccg gacgagattg gctgctgctg ggagaagtgc caggacagga cctgctgagc
     8221 tcccacctgg caccagctga gaaggtctcc atcatggcag atgccatgag gagactgcat
     8281 actctggacc ccgccacctg ccctttcgat caccaggcta aacatcgcat tgagcgggct
     8341 cgcacacgaa tggaagcagg cctggtggac caggacgatc tggatgagga acaccaggga
     8401 ctggctcctg cagagctgtt tgcaaggctg aaagccagaa tgccagacgg cgaagatctg
     8461 gtggtcaccc atggagacgc ttgcctgccc aacatcatgg tggagaatgg aagattcagt
     8521 gggtttattg attgtggccg actgggagtc gccgacaggt accaggatat cgccctggct
     8581 acaagagaca ttgcagagga actgtgcggg gaatgggccg atcggttcct ggtgctgtat
     8641 ggcattgctg ctcccgacag tcagaggatt gctttttaca ggctgctgga cgagttcttt
     8701 tgaatctaga cgcgtatcga tnnatcgata agcttagatc gagcacaaga ggaagagaga
     8761 gaccctcact gctggggagt ccctgccaca ctcagtcccc caccacactg aatctcccct
     8821 cctcacagtt gccatgtaga ccccttgaag aggggagggg cctagggagc cgcaccttgt
     8881 catgtaccat caataaagta ccctgtgctc aaccagttac ttgtcctgtc ttattctagg
     8941 gtctggggca gaggggaggg aagctgggct tgtgtcaagg tgagacattc ttgctgggga
     9001 gggacctggt atgttctcct cagactgagg gtagggcctc caaacagcct tgcttgcttc
     9061 gagaaccatt tgcttcccgc tcagacgtct tgagtgctac aggaagctgg caccactact
     9121 tcagagaaca aggccttttc ctctcctcgc tccagtccta ggctatctgc tgttggccaa
     9181 acatggaaga agctattctg tgggcagccc cagggaggct gacaggtgga ggaagtcagg
     9241 gctcgcactg ggctctgacg ctgactggtt agtggagctc agcctggagc tgagctgcag
     9301 cgggcaattc cagcttggcc tccgcagctg tgaggtcttg agcacgtgct ctattgcttt
     9361 ctgtgccctc gtgtcttatc tgaggacatc gtggccagcc cctaaggtct tcaagcagga
     9421 ttcatctagg taaaccaagt acctaaaacc atgcccaagg cggtaaggac tatataatgt
     9481 ttaaaaatcg gtaaaaatgc ccacctcgca tagttttgag gaagatgaac tgagatgtgt
     9541 cagggtgact tatttccatc atcgtcctta ggggaacttg ggtaggggca aggcgtgtag
     9601 ctgggaccta ggtccagacc cctggctctg ccactgaacg gctcagttgc tttgggcagt
     9661 tactcccggg cctcactttg cacgtgtgct tacctagtgg agacaaaagt acatacctcg
     9721 gtagagcgcg cacgcctgta accccagcac tttgggaggc caaggtgggt gtatcacctg
     9781 aggtcaggag tttgagacca gcctggccaa catggtgaaa ctccgtctct actaaaatta
     9841 caaaaatcag ccaggcttca tggcacatgc ctatagtccc agctacaggc atgctgaagc
     9901 aggagaatcg cttgcacccc ggaggcagag gctgcagtga gctgagacca caccactgca
     9961 ctccagccta ggcaacagag tatgagactc catctcaaaa aaaaaaaaag tacctacctc
    10021 agagttcaaa ctagtgaata ttaggaagtg cttgagacag tgacaccaaa gtgcacaata
    10081 aatactcgcc agtttcatta ttattaaaga atccatttga atgtcagctc aacacagcct
    10141 cctataccga ggcattgtga accgcatctc cccagcttct ccaggctttt ccaagaatca
    10201 gggacactgt agcctgttgg tctcagtgta tgacagacac ggaggaagca catctttagc
    10261 tgatacttaa acagagaccc tgagcgcaca tacacccgcg cacacatgca tggagcttca
    10321 ccttctctgt cattctgcag tgaccaggag agcaagagct cccacctccc ttcaaaacac
    10381 tgtgcccatc ccgggcacta aggcctcttt aaagcacggc acctccacga gggagggcca
    10441 cagccacata cactccacct ggcaggtgga cagcgtgagc acgtggacca tagcagggac
    10501 aaggtgcccc ggccagcccc aacgccctct gccgctgaca gggacagaag ccctctccag
    10561 ctgcgtgtgc tgcagaggcc atgcgtagcc tccagctgca ttctattcca ctccagtgcc
    10621 tgggccagtt agcaccagtg tggaagacag tgagctggct ccggacaaca gggatggagg
    10681 aaaggtccca cattcacatt cctgatacgt ggacaaggtg aggggccgca atcgctctgg
    10741 cagcatttta aagatgggga agtagcagac acccacgcgt gaaggcagga gagccccaac
    10801 tgtggtggaa atggccccag aatggtaggg ccaagcctag ctccagacac cccagagccc
    10861 tggagaagcc aagactgagg gagaaagcct gagggaggag cgccccagtc cccagggacc
    10921 ggcctggtgc agagctgcag ctgatgttcc cctctgtgca gccccaccct ctgcctcgct
    10981 gagctccctg ctgcgagggc ctcgggtgca agggggaggc aggtctctat ctcatggagc
    11041 tgtcagatga gacatcgcga tcggagtcct cagcctcgct tggcggcggc ggcgggtcgc
    11101 taagcgggac cgcagtgaaa gcaggagact ttctagaaaa aaacaccagt tgtcaacctt
    11161 ggggcaggca ggaatcctga agacggacgg cactcctcct cctgctgcct caccctctgg
    11221 cagcccgtga gaagtaccgg aagcgagggc ggggccgcgg gatggcgagg gagcggcagg
    11281 gactgaactc tctccaaacc caccctgaca gggaaatggg ccccgcctgt gtcttgggaa
    11341 ctcagaggct gaggtcaggc atgatggctc acgcctgtaa ttccagcact ttgggaggca
    11401 gaggcgggtg gatcacgacg tcaggagttc aaggcaagcc tggccaacat ggtgaaaccc
    11461 catctctact aaaaatgcaa aaattagcca ggtgtggtgg cgggcgcctt ggaggctgag
    11521 gcaagataat cacttgaacc tgggaggcgg aggttgtagt gagccaaaaa aaaaaaaaaa
    11581 agaaatagct gaagtcacag taggagagaa gctgctgagc ctccagcacc ctgactctag
    11641 ggccttggct ttatgtctat ctgcagtatt tttgtgattt ttaaaaattc actttcttgt
    11701 tgcggtgtaa cttacacagg gtcaaatgca caaatcatgg ccctgacttt agataaaaat
    11761 ctgcccccac aacccttctg ttccttgcca gtttttaaac tgcctctaac caggggaacc
    11821 accagagctg gtggccttgg gaggtttcag ccctcccgtc atgaatggac atagctcatc
    11881 caactgccaa gggagagagc tgtgggtctg ggccagcccc accaggtaac tcccaaaggg
    11941 cagccccaca gcaagatgtg acccagtcat tgcctgaggg tctctggggc tgtgttccaa
    12001 cctttctccc cgctgtgtcc ccctggaagg ccccatgccc aggggaggcg cctaccggtc
    12061 tccagactgg gtgatgagcc ggcggcaggt ctccatctgc acgtccaggc cgcgcttcat
    12121 gctgcacatc tccatgtact cgtgcaggtg ccggttcatg tcgttcttgg ccgtggccaa
    12181 ctccagctgt ggtggggcag gcagggccat cggggttaac aggtggcgtt cacagcgcct
    12241 ctgttgcccc cgccaggagg ccaacacgcc aagagcagtg gctgggccgg gggcccaggc
    12301 agccattact gaaggctcag attttaaaat aaaccagacc aaggcagaag gcagcgaata
    12361 caccattgtt tcctctgctt ttcctggaaa tctttgccta tggaggaatg caaaggtact
    12421 gctgcaggct gtgttctttt aagactgata agagtggtga ggaaagtcag tgtgggcggg
    12481 atggagcaga tccacaggct cccagggcca cacaggttcc tatggggccc ggctcacccc
    12541 tcatcccctg tactgcgctg gtcctgggct gtcccctcac tgcactgtga gctcccgtaa
    12601 gggttagcga caggttttcc tcatcctggc agacccagac ctcgaaggga attaactctc
    12661 tctgtgaccc gtggcataca agcctggcta actgctgaaa ggggcccaat cctcttacca
    12721 ttctctcctt atcactttat tttctcacct aaaagccatt ttcagacact aaaaggaaga
    12781 ctcccatatt ggaggtgcca ttgattctga gacctgtcct gatttcagaa atgtcacacg
    12841 tggaaaacac ggggctcaga atcaaagaaa tccggtctcc ctccccgggc ccattctgca
    12901 gcccacactc tgaggcaggc ggagaggcag ggcccaggca gcacaaactc agtgccaggg
    12961 acagtcgatt aattaagcga tcgcaggccg cccgcggtgg agctccaatt cgccctatag
    13021 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    13081 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    13141 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
    13201 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    13261 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    13321 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    13381 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    13441 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    13501 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    13561 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    13621 cccgccgcgc ttaatgcgcc gctacagggc gcgtcag
//
