LOCUS       sequence-156582-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CB_p.A1048V
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             276..284
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
       61 tggcaccatg tgcttcagtt tcataatgcc tcctgctatg gcagacatcc ttgacatctg
      121 ggcggtggat tcacagatag catctgatgg ctccatacct gtggatttcc ttttgcccac
      181 tgggatttat atccagttgg aggtacctcg ggaagctacc atttcttata ttaagcagat
      241 gttatggaag caagttcaca attacccaat gttcaacctc cttatggata ttgactccta
      301 tatgtttgca tgtgtgaatc agactgctgt atatgaggag cttgaagatg aaacacgaag
      361 actctgtgat gtcagacctt ttcttccagt tctcaaatta gtgacaagaa gttgtgaccc
      421 aggggaaaaa ttagactcaa aaattggagt ccttatagga aaaggtctgc atgaatttga
      481 ttccttgaag gatcctgaag taaatgaatt tcgaagaaaa atgcgcaaat tcagcgagga
      541 aaaaatcctg tcacttgtgg gattgtcttg gatggactgg ctaaaacaaa catatccacc
      601 agagcatgaa ccatccatcc ctgaaaactt agaagataaa ctttatgggg gaaagctcat
      661 cgtagctgtt cattttgaaa actgccagga cgtgtttagc tttcaagtgt ctcctaatat
      721 gaatcctatc aaagtaaatg aattggcaat ccaaaaacgt ttgactattc atgggaagga
      781 agatgaagtt agcccctatg attatgtgtt gcaagtcagc gggagagtag aatatgtttt
      841 tggtgatcat ccactaattc agttccagta tatccggaac tgtgtgatga acagagccct
      901 gccccatttt atacttgtgg aatgctgcaa gatcaagaaa atgtatgaac aagaaatgat
      961 tgccatagag gctgcc
//
