LOCUS       sequence-156593-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector that expresses the HIV-1 GAG protein 
            with HRAS fused to it.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, 
            Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van 
            Calenbergh S, Gevaert K, Tavernier J
  TITLE     Trapping mammalian protein complexes in viral particles.
  JOURNAL   Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416.
  PUBMED    27122307
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-28"; volume: "7:11416. doi"; pages: " 
            10.1038/ncomms11416"
COMMENT     Sequence Label: pMET7-GAG-HRAS
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagctgcggg cccgctagcg ccaccatggg tgcgagagcg tcagtattaa gcgggggaga
       61 attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      121 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      181 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      241 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      301 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      361 gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca gccaaaatta
      421 ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac ctagaacttt
      481 aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga tacccatgtt
      541 ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa acacagtggg
      601 gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcagaatg
      661 ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga gagaaccaag
      721 gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat ggatgacaca
      781 taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg gattaaataa
      841 aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac caaaggaacc
      901 ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag cttcacaaga
      961 ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag attgtaagac
     1021 tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag catgtcaggg
     1081 agtggggg
//
