LOCUS       sequence-156600-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector that expresses HIV-1 GAG with EGFP fused
            to it.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Eyckerman S, Titeca K, Van Quickelberghe E, Cloots E, Verhee A, 
            Samyn N, De Ceuninck L, Timmerman E, De Sutter D, Lievens S, Van 
            Calenbergh S, Gevaert K, Tavernier J
  TITLE     Trapping mammalian protein complexes in viral particles.
  JOURNAL   Nat Commun. 2016 Apr 28;7:11416. doi: 10.1038/ncomms11416.
  PUBMED    27122307
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-28"; volume: "7:11416. doi"; pages: " 
            10.1038/ncomms11416"
COMMENT     Sequence Label: pMET7-GAG-EGFP
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      987..996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1038..1059)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atcaagcagc catgcaaatg ttaaaagaga ccatcaatga ggaagctgca gaatgggata
       61 gagtgcatcc agtgcatgca gggcctattg caccaggcca gatgagagaa ccaaggggaa
      121 gtgacatagc aggaactact agtacccttc aggaacaaat aggatggatg acacataatc
      181 cacctatccc agtaggagaa atctataaaa gatggataat cctgggatta aataaaatag
      241 taagaatgta tagccctacc agcattctgg acataagaca aggaccaaag gaacccttta
      301 gagactatgt agaccgattc tataaaactc taagagccga gcaagcttca caagaggtaa
      361 aaaattggat gacagaaacc ttgttggtcc aaaatgcgaa cccagattgt aagactattt
      421 taaaagcatt gggaccagga gcgacactag aagaaatgat gacagcatgt cagggagtgg
      481 ggggacccgg ccataaagca agagttttgg ctgaagcaat gagccaagta acaaatccag
      541 ctaccataat gatacagaaa ggcaatttta ggaaccaaag aaagactgtt aagtgtttca
      601 attgtggcaa agaagggcac atagccaaaa attgcagggc ccctaggaaa aagggctgtt
      661 ggaaatgtgg aaaggaagga caccaaatga aagattgtac tgagagacag gctaattttt
      721 tagggaagat ctggccttcc cacaagggaa ggccagggaa ttttcttcag agcagaccag
      781 agccaacagc cccaccagaa gagagcttca ggtttgggga agagacaaca actccctctc
      841 agaagcagga gccgatagac aaggaactgt atcctttagc ttccctcaga tcactctttg
      901 gcagcgaccc ctcgtcacaa ggcgggtcga cgagctcaga attctgcagt cgacggtacc
      961 gcgggcccgg gatccaccgg ccggtcgcca ccatggtgag caagggcgag gagctgttca
     1021 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttca
//
